Milli q water purification apparatus

Manufactured by Merck Group

Sourced in United States

The Milli-Q water purification apparatus is a laboratory equipment designed to produce high-purity deionized water. It utilizes a multi-stage filtration process to remove impurities, resulting in water that meets the stringent quality standards required for various scientific and industrial applications.

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8 protocols using milli q water purification apparatus

Synthesis and Characterization of Methylated Nucleosides

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Chromatographic grade acetonitrile was bought from Merck KGaA (Darmstadt, Germany). Formic acid was obtained from Fluka (Muskegon, MI, USA) and water was obtained from a Milli-Q water purification apparatus (Millipore, Milford, MA, USA). Malic acid, ammonium formate and m⁵C were bought from Sigma-Aldrich (St Louis, MO, USA). Nine other methylated nucleosides, [D₃]m⁶A, [D₃]m¹A, [D₃]m⁶A_m, [D₃]U_m, [¹³C₅]m⁵U, [¹³C¹⁵N₂]G and [¹³C₅]C, were purchased from Toronto Research Chemicals (Toronto, Canada); [¹³C₅]A_m and [¹³C₅]m⁵C were synthesized previously [3 (link),4 (link)].

Fang Z., Hu Y., Hong X., Zhang X., Pan T., Pan C., Zheng S, & Guo C. (2022). Simultaneous Determination of Methylated Nucleosides by HILIC–MS/MS Revealed Their Alterations in Urine from Breast Cancer Patients. Metabolites, 12(10), 973.

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Quantitative Ginsenoside Analysis Protocol

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Authentic ginsenoside standards with more than 98% purity were purchased from Shanghai Yuanye Biological Technology Co. Ltd. (Shanghai, China) and used without further purification. The intermediate biotransformation products of PPD-type ginsenosides were provided by Professor Yue of our laboratory. HPLC-grade methanol was acquired from Tedia (Fairfield, OH, USA). Distilled water was purified using a Milli-Q water purification apparatus (Millipore, Bedford, MA, USA). The ginsenosides were accurately weighed and dissolved in a methanol/water (1:1, v/v) solvent mixture to obtain a final concentration of 0.1 nmol μL⁻¹. The solutions were filtered through a 0.45 μm membrane and then subjected to MS analyses.

Xiu Y., Ma L., Zhao H., Sun X., Li X, & Liu S. (2017). Differentiation and identification of ginsenoside structural isomers by two-dimensional mass spectrometry combined with statistical analysis. Journal of Ginseng Research, 43(3), 368-376.

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Purification and Analytical Techniques for Natural Products

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Data for specific rotation measurements were obtained on a Rudolph Research Autopol III automatic polarimeter. Column chromatography was performed using silica gel and HP20SS. Preparative HPLC was carried out on a Shimadzu system equipped with LC-6AD pumps, coupled to an SPD-M20A PDA detector, and a Phenomenex Luna C₁₈ column (21.2 × 250 mm and 10 × 250 mm, 5 μm). Analytical and semipreparative HPLC were conducted using a Waters HPLC system with 1525 binary pumps and a 2998 PDA detector using Phenomenex Gemini C₁₈ (250 × 4.6 mm, 1 mL/min, 5 μm) and Kinetex pentafluorophenyl (250 × 10 mm, 4 mL/min, 5 μm) columns. NMR data were collected on a Varian 600 MHz NMR spectrometer. Microcentrifuge-tube-based ultrafiltration filters (100 kDa) were obtained from Pall Corporation (Houston, TX, USA). Salmon sperm DNA and other chemicals were purchased from MilliporeSigma (St. Louis, MO, USA). Cephaeranthine was purchased from Cayman Chemical Company (Ann Arbor, MI, USA), and tetrandrine (16) and daurisoline (17) were obtained from MilliporeSigma. Distilled water was prepared with a Milli-Q water purification apparatus (Millipore, Bedford, MA, USA). All solvents were of ACS grade or better.

Ma H., Liang H., Cai S., O’Keefe B.R., Mooberry S.L, & Cichewicz R.H. (2020). An Integrated Strategy for the Detection, Dereplication, and Identification of DNA-Binding Biomolecules from Complex Natural Product Mixtures. Journal of natural products, 84(3), 750-761.

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HPLC Analysis of 8-oxodG Oxidative Damage

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Chromatographic grade methanol (MeOH) used for HPLC was purchased from Merck (Darmstadt, Germany). 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), acetic acid (CH₃COOH), were purchased from Sigma-Aldrich (St Louis, MO, USA). Isotopically labeled internal standard (IS), [¹⁵N₅]8-oxo-7,8-dihydro-2′-deoxyguanosine ([¹⁵N₅]8-oxodG) was purchased from Cambridge Isotope Laboratories Inc. (Andover, MA, USA). Water used throughout all experiments was purified using a Milli-Q water purification apparatus (Millipore, Milford, MA, USA). 8-oxodG and [¹⁵N₅]8-oxodG were dissolved in water to a stock concentration of 1 mM and 0.1 mM, respectively. Aliquots were stored at −80°C until use. When required, these were diluted to 1 μM in water.

Guo C., Li X., Ye M., Xu F., Yu J., Xie C., Cao X., Guo M., Yuan Y, & Zheng S. (2017). Discriminating patients with early-stage breast cancer from benign lesions by detection of oxidative DNA damage biomarker in urine. Oncotarget, 8(32), 53100-53109.

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Quantification of 8-OHdG and 8-OHG

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Methanol (MeOH) of HPLC grade was obtained from Merck KGaA (Darmstadt, Germany). Formic acid (HCOOH), acetic acid (CH₃COOH), and 8-OHdG were bought from Sigma-Aldrich (St Louis, MO, USA). [¹⁵N₅]8-Hydroxy-2′-deoxyguanosine ([¹⁵N₅]8-OHdG) was purchased from Cambridge Isotope Laboratories Inc. (Andover, MA, USA). 8-OHG and [¹³C¹⁵N₂]8-hydroxyguanosine ([¹³C¹⁵N₂]8-OHG) were bought from Toronto Research Chemical (Toronto, Canada). Water was gained from a Milli-Q water purification apparatus (Millipore, Milford, MA, USA).

Chen Q., Hu Y., Fang Z., Ye M., Li J., Zhang S., Yuan Y, & Guo C. (2020). Elevated Levels of Oxidative Nucleic Acid Modification Markers in Urine From Gastric Cancer Patients: Quantitative Analysis by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry. Frontiers in Chemistry, 8, 606495.

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HPLC-MS/MS Method for 8-OHdG Quantification

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HPLC grade methanol (MeOH), 8-hydroxy-2′-deoxyguanosine (8-OHdG), 2′-deoxyguanosine (dG), formic acid (HCOOH), acetic acid (CH₃COOH), ammonium formate (HCOONH₄) ammonium acetate (CH₃COONH₄), ammonium bicarbonate (NH₄HCO₃) were purchased from Sigma-Aldrich (St Louis, MO, USA). Water used throughout all experiments was purified using a Milli-Q water purification apparatus (Millipore, Milford, MA, USA). Isotopically labeled internal standard (IS), [¹⁵N₅]8-hydroxy-2′-deoxyguanosine ([¹⁵N₅]8-OHdG) was purchased from Cambridge Isotope Laboratories Inc. (Andover, MA, USA). 8-OHdG and [¹⁵N₅]8-OHdG were reconstituted in water to a stock concentration of 1 mM and 0.1 mM, respectively. Aliquots were stored at −80 °C until required. When required, these were diluted to 1 μM in water.

Guo C., Li X., Wang R., Yu J., Ye M., Mao L., Zhang S, & Zheng S. (2016). Association between Oxidative DNA Damage and Risk of Colorectal Cancer: Sensitive Determination of Urinary 8-Hydroxy-2′-deoxyguanosine by UPLC-MS/MS Analysis. Scientific Reports, 6, 32581.

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Quantification of Nucleoside Modifications

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Chromatographic-grade methanol was obtained from J.T. Baker (Radnor, PA, United States). Acetonitrile of HPLC grade was bought from Merck KGaA (Darmstadt, Germany). A, m⁶A, m¹A, Am, m⁶Am, m⁶₂A, ¹³C₅-adenosine ([¹³C₅]A), D₃-N⁶-methyladenosine ([D₃]m⁶A), D₃-N¹-methyladenosine ([D₃]m¹A), and D₃-N⁶-2′-O-dimethyladenosine ([D₃]m⁶Am were gained from Toronto Research Chemical (Toronto, ON, Canada). Acetic acid (CH₃COOH) was purchased from Fluka (Muskegon, MI, United States). Ammonium acetate and malic acid were bought from Sigma Aldrich (St Louis, MO, United States). Water was purified by using a Milli-Q water purification apparatus (Millipore, Milford, MA, United States).

Hu Y., Fang Z., Mu J., Huang Y., Zheng S., Yuan Y, & Guo C. (2021). Quantitative Analysis of Methylated Adenosine Modifications Revealed Increased Levels of N6-Methyladenosine (m6A) and N6,2′-O-Dimethyladenosine (m6Am) in Serum From Colorectal Cancer and Gastric Cancer Patients. Frontiers in Cell and Developmental Biology, 9, 694673.

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HPLC-Based Quantification of Polygonumnolide Dianthrones

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HPLC-grade acetonitrile was purchased from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid was purchased from Merck Inc. (Darmstadt, Germany). Ethanol was of analytical grade and purchased from Shanghai Chemical Reagent (Shanghai, China). Water was purified by a Milli-Q water purification apparatus (Millipore, Billerica, MA, USA). The immortalized hepatic cell line HepaRG was obtained from the Type Culture Collection of the Chinese Academy of Sciences, (Shanghai, China), RPMI 1640 culture medium (Biological Industries, Israel), Fetal Bovine Serum (Biosera, France), penicillin (Targetmol, China), Staurosporine ( STSP,Targetmol, China), 0. The chemical requirements for Polygonumnolide C4 (1), Polygonumnolide C3 (2), Polygonumnolide C1 (3), Polygonumnolide C2 (4), transemodin dianthrones (5) , and cis-emodin dianthrones (6) were isolated and purified. The structures of the six dianthrones (1-6) were confirmed by UV, MS, 1 HNMR and 13 C NMR analysis, which has been reported in the literature [13] [14] [15] . The purity of these compounds was more than 98.0% (determined by HPLC). The internal standard (IS) (Bianthronyl, IS) was purchased from Moving Your Chemistry Forward (Shanghai, China). Fig. 1 shows the six dianthrones structures and one IS. All solvents and samples were filtered through 0.22μm filters before injection into UHPLC.

Yang J., Song Y., Liu Y., Gao H., Wang Q., Wang Y., Cheng X., Zuo T., Hu X., Feng W., Jin H., wang S., & Ma S. (2021). UHPLC-QQQ-MS/MS assay for quantification of dianthrones as potential toxic markers of Polygonum multiflorum Thunb: Application to the standardization of TCMs with endogenous toxicity.

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