Sybr premix ex taq 2 enzyme kit

GuangZao and RouDouKou were purchased from Huhehaote Medicinal Company, (Huhehaote, China). The FCN herb pair (GuangZao and RouDouKou) is prepared at a ratio of 1:1. A TRIzol reagent Kit, SYBR® Premix Ex Taq™ II enzyme kit, and PrimeScript™RT Master Mix RT were purchased from Takara Bio, Inc. (Dalian, China), and a Total RNA Extraction Kit was purchased from Tiangen Biotech Co., Ltd. (Beijing, China).

Antibodies included rabbit anti-Akt1 (1:1000, CST: #75,692, USA), rabbit anti-p-Akt1 (1:1000, CST: #9018, USA), rabbit anti-FoxO3a (1:1000, CST: #2497, USA), rabbit anti-p-FoxO3a (1:1000, CST: #9466, USA), rabbit anti-Bim (1:1000, CST: #2933, USA), rabbit anti-GAPDH (1:1000, CST: #2118, USA) and goat anti-rabbit secondary antibody (1:3000, Bioss biotechnology Co. LTD., China). Others included Total RNA isolation kit (Tiangen Biotech Co., LTD., China), TRIzol reagent Kit, SYBR Premix Ex Taq II enzyme kit, and PrimeScriptRT Master Mix (Takara Bio, Inc., China), Evans blue, 2,3,5-triphenyltetrazolium chloride (TTC, Solarbio Technology, China), LY294002 (PI3K inhibitor, Sigma), Lactate dehydrogenase (LDH) and Creatine kinase (CK) Kits (MedicalSystem Biotechnology CO. Ltd, China), goat anti-rabbit IgG/FITC (Beijing biosynthesis biotechnology CO.Ltd, China), Annexin V-FITC Apoptosis Detection Kit(Beyotime biotechnology, China) and BCA protein Assay kit (Merck Millipore Technology, Germany). Oligo synthesis was performed by Sangon Biotech co. LTD (Shanghai, China).