Anti uchl5

Chemicals used were: bortezomib (LC Laboratories), Propidium Iodide and N-acetylcysteine (Sigma Aldrich). Primary antibodies used were: anti-Gas2^{36 (link)} anti-actin (Sigma/Aldrich) anti-USP1^{37 (link)} anti-USP33 (Millipore) anti-AKT (Cell Signaling) anti-UCH-L5 (Abcam), anti-USP18^{38 (link)} anti-HDAC4^{39 (link)} anti-MEF2D (BD Bioscience), anti-GRP78, anti-USP14, anti-Cofilin-1, anti-pCofilin-1 (Santa Cruz Biotechnologies).

NiPT, PtPT, PdPT, T-AuPT, Au(PPh₃)PT, CuPT, and AgDT were synthesized in our laboratory and stored as a 10 mM stock solution in dimethyl sulfoxide (DMSO) at −20°C. Auranofin was purchased from Enzo Life Sciences International, Inc. (Plymouth Meeting, PA) and dissolved in DMSO as a 10 mM, stored at −20°C. Bortezomib (Cell Signaling Technology, Beverly, MA, USA) HA–ubiquitin–vinyl sulfone (HA-Ub-VS), and Ub-AMC (BostonBiochem, Cambridge, MA, USA). Antibodies were purchased from following sources: anti-AMPK, anti- phospho-AMPK, anti-mTOR, anti-phospho-mTOR, anti-P70S6K, anti-phospho-P70S6K, anti-S6, anti-phospho-S6, anti-ATF4, anti-CHOP, anti-phospho-eIF2a, anti-LC3, anti-USP14 (D8Q6S), and anti-PARP (Cell Signaling Technology, Beverly, MA, USA); anti-ubiquitin (P4D1) and anti-SQSTM1/P62(Santa Cruz Biotechnology, Santa Cruz, CA); anti-GAPDH and anti-HA-tag (Bioworld Technology, Nanjing, China); and anti-UCHL5 (Abcam, Cambridge, UK). Anti-phospho-USP14 and anti-phospho-UCHL5 were prepared by the Abgent, Inc. Bafilomycin, 3-MA, and E64D were purchased from Enzo Life Sciences (MA, USA). Lipofectamine 2000 (11668–027), goat anti-rabbit Alexa 488 (997773), and opti-MEM medium (22600050) were purchased from the Invitrogen^TM.