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Mouse anti β actin

Manufactured by R&D Systems
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The Mouse anti-β-actin is a primary antibody that recognizes and binds to the β-actin protein, which is a ubiquitous and highly conserved cytoskeletal protein found in eukaryotic cells. This antibody can be used in various applications, such as Western blotting, immunocytochemistry, and immunohistochemistry, to detect and quantify the expression levels of β-actin.

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Lab products found in correlation

Rabbit anti erk1 2, by Cell Signaling Technology (1 mentions) Clarity western ecl substrate chemiluminescence kit, by Bio-Rad (1 mentions) Rabbit anti p erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Chemidoc xrs imaging acquiring system, by Bio-Rad (1 mentions) Image lab software v 6, by Bio-Rad (1 mentions) Mouse anti gapdh, by GeneTex (1 mentions) Anti gapdh, by Thermo Fisher Scientific (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti asymmetric dimethyl arginine antibodies, by Active Motif (1 mentions) Anti h4r3me2a antibodies, by Active Motif (1 mentions)

3 protocols using mouse anti β actin

1

Western Blot Analysis Optimization

Cited 1 time
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Cells extraction, lysate quantification, SDS-Page and western blots were performed as described using stain-free gels [20 (link)]. Membranes were incubated with rabbit anti-β-catenin, mouse anti-GAPDH (GeneTex), goat anti-pGsk-3β (Ser9), rabbit anti-LaminB1, mouse anti-pCREB (Ser133), mouse anti-pMEK1/2, mouse anti-β-actin, rabbit anti-FAK, rat anti-pFAK (Y397, R&D Systems) rabbit anti-PI3K, goat anti-pPI3K (Tyr 508), goat anti-AKT1, rabbit anti-pAKT1 (Tyr308), rabbit anti-ERK1/2 (Cell Signaling Technology, Frankfurt am Main, Germany), rabbit anti pERK1/2 (Thr202/Tyr204, Cell Signaling Technology) as well as goat anti-rabbit, goat anti-rat, donkey anti-goat and anti-mouse IgG kappa binding protein IgG HRP-conjugated diluted in 1% BSA solution. If not indicated otherwise antibodies were purchased from Santa Cruz Biotechnology. Western blots were quantified via chemiluminescence using a Clarity Western ECL substrate chemiluminescence kit (BioRad). Besides the loading control β-Actin, we used stainfree total protein normalization. Membranes were photographed and quantified using ChemiDoc XRS+ imaging acquiring system (BioRad) and Image Lab software v. 6.0 (BioRad).
Jakubzig B., Baltes F., Henze S., Schlesinger M, & Bendas G. (2018). Mechanisms of Matrix-Induced Chemoresistance of Breast Cancer Cells—Deciphering Novel Potential Targets for a Cell Sensitization. Cancers, 10(12), 495.
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2

SDS-PAGE and Immunodetection of Cubilin

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SDS-PAGE and immunodetection of cubilin were performed essentially as described elsewhere [23 (link)]. For detection of β-actin, protein samples were separated by 10% SDS-PAGE. Antibodies: sheep anti-cubilin (cat. # AF3700, R&D Systems, Abingdon, United Kingdom), 1.0 µg/mL; mouse anti-β-actin (cat. # A5316, Sigma-Aldrich, Taufkirchen, Germany), 1:20,000.
Fels J., Scharner B., Zarbock R., Zavala Guevara I.P., Lee W.K., Barbier O.C, & Thévenod F. (2019). Cadmium Complexed with β2-Microglubulin, Albumin and Lipocalin-2 rather than Metallothionein Cause Megalin:Cubilin Dependent Toxicity of the Renal Proximal Tubule. International Journal of Molecular Sciences, 20(10), 2379.
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3

Antibody Characterization for Epigenetic Proteins

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Anti-PRMT1 (F339), Acetyl-Histone H3 (Lys27) (D5E4), anti-CyclinD and anti-β-catenin antibodies were from Cell Signaling. Anti-Lamin B (C20), anti-β-actin were from Santa Cruz. Rabbit Anti-PRMT1 antibody (against aa 300-361), were from Abcam. Anti-asymmetric-dimethyl-arginine antibodies and Anti-H4R3me2a antibodies were from ActiveMotif. Monoclonal anti-HNF4α (H1415) antibodies were from R&D Systems Inc. Mouse anti- β-actin, mouse Monoclonal Anti-PRMT1 clone 171 (against aa 1-361), Anti-JMJD6 (SAB3500233) antibodies were from Sigma-Aldrich, Saint Louis, MO. Anti-GAPDH was from Ambion.
Zhao J., Adams A., Roberts B., O'Neil M., Vittal A., Schmitt T., Kumer S., Cox J., Li Z., Weinman S.A, & Tikhanovich I. (2018). PRMT1 and JMJD6 dependent arginine methylation regulate HNF4α expression and hepatocyte proliferation. Hepatology (Baltimore, Md.), 67(3), 1109-1126.
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