Victor multilabel counter 1420 spectrophotometer

The biocompatibility of fabricated electrospinning was assessed for culturing and proliferation of SCs by the colorimetric MTS assay (CellTiter 96 Aqueous One Solution Cell Proliferation Assay, Promega, Madrid, Spain). Cell proliferation was determined by a tetrazolium compound (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS assay) after 1, 3, and 7 days of cell culture. The cells were washed with sterile PBS to avoid color interference from the compound and then incubated for 3 h with MTS reagent. Finally, the medium was removed and its absorbance was measured at 490 nm in a plate reader (Victor Multilabel Counter 1420 spectrophotometer, Perkin Elmer, Madrid, Spain).

To evaluate the cell proliferation inside conduits, MTS assays (CellTiter 96 Aqueous One Solution Cell Proliferation Assay, Promega, Madrid, Spain) were carried out on the unimodular and multimodular conduits (n = 5 each). When incorporated into the cells, the MTS was reduced by metabolically active cells at 5 days of SC cell culture in a rate proportional to the number of live cells. After 1 h of incubation with the MTS reagent, the medium was removed, and its absorbance was measured with a Victor Multilabel Counter 1420 spectrophotometer (Perkin-Elmer, Madrid, Spain) at 490 nm.