Iron chloride fecl2

Cell culture media and supplements, Lipofectamine RNAiMAX and Lipofectamine 3000 were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). 4-Amino-5-methylamino-2,7-difluorofluorescein (DAF-FM) diacetate and N⁵-(1-iminoethyl)-L-ornithine HCl (L-NIO) were obtained from Molecular Probes (Eugene, OR, USA) and Alexis Biochemicals (San Diego, CA, USA), respectively. Antibodies against human eNOS and HO-1 were purchased from BD Biosciences (San Jose, CA, USA). Other antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). miRNAs, siRNA and miRNA assay chemicals were purchased from QIAGEN (Hilden, Germany). Luciferase reporter assay kits were obtained from Promega (Madison, WI, USA). The TNF-α and a cGMP assay kits were purchased from R&D Systems (Minneapolis, MN, USA). Oxyhemoglobin (oxyHb) was prepared by reduction of human hemoglobin (Sigma-Aldrich, St. Louis, MO, USA) with a 20-fold excess amount of sodium dithionite for 20 min, followed by gel filtration using a pre-packed disposable column (PD-10, Pharmacia, Uppsala, Sweden) that had been pre-equilibrated with 50 mM Tris-HCl (pH 7.4). Sn(IV) protoporphyrin IX dichloride (SnPP) and biliverdin were obtained from Frontier Scientific (Logan, UT, USA). RuCl₃, CORM-2, hemin, bilirubin and iron chloride (FeCl₂) were purchased from Sigma-Aldrich.