Doxorubicin

PPI was purchased from the Institute for Drug Control (Shanghai, China, #111590) and dissolved in dimethyl sulfoxide (DMSO). Doxorubicin was purchased from Sangon Biotech (Shanghai, China, #DB3456) and dissolved in ultra-purified water. FITC Annexin V and propidium iodide were obtained from BD Biosciences (Franklin Lakes, USA, #556420). The cell cycle detection kit was from Key GENBioTECH (Nanjing, China). Antibodies against PARP (#9542), BAX (D2E11, #5023), BCL-2 (#2876), Vimentin (5G3F10, #3390), C-Myc (D84C12, #5605), GSK-3β (27C10, #9315), phosphor-GSK-3β (#8213), active β-catenin (D13A1, #8814) and β-catenin siRNA II (#6238) were from Cell Signaling Technology (Danvers, USA). Trypsin-EDTA, Lipofectamine 2000 (#11668) and TRIzol reagent (#15596) were from Invitrogen (Rockville, MD, USA). CHIR99021 (#SML1046) and antibody against β-actin (#A2228) were from Sigma-Aldrich (Saint Louis, USA). Monoclonal antibody of rabbit anti-Ki-67 and RIPA Cell Lysis Buffer (#P0013B) were got from Beyotime Institute of Biotechnology (Shanghai, China). SuperSignal Chemiluminescent HRP Substrate (#34078) was from Thermo Fisher scientific Inc (Rockford, USA). Matrigel (#356234) was purchased from BD Biosciences (Bedford, USA). PrimeScript RT reagent kit (#RR037A) and SYBR Premix Ex Taq II (#RR420L) were bought from TaKaRa (Dalian, China).

Graphite powder was purchased from Acros. KMnO₄ (AR), NaNO₃ (AR), linear chain PEG amine (5000), branched polyethylenimine 10000 (BPEI 10000), folic acid, EDC and NHS, were purchased from Sigma-Aldrich. Doxorubicin was purchased from Sangon Biotech (Shanghai, China). Modified RPMI-1640 Medium was purchased from HyClone. Fetal bovine serum (FBS), penicillin-streptomycin, and trypsin were obtained from Gibco. Propidium iodide, LysoTracker, MitoTracker and Hoechst were purchased from Molecular Probes (USA). The Cell Counting Kit-8 (CCK-8) and Calcein AM were purchased from Dojindo (Japan). The β-actin antibody was purchased from GeneTex (USA). The dialysis bags and ultrafiltration tubes (molecular weight cutoff: 10-kDa and 30-kDa) were purchased from Millipore. The P-gp siRNA, the scrambled siRNA and FAM-labelled siRNA were supplied by GenePharma (Shanghai, China).

Sangon Biotech (Shanghai, China) provided the following materials for our subject paper: a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) kit, doxorubicin (DOX), phenylmethylsulfonyl fluoride (PMSF), and 4′,6-diamidino-2-phenylindole (DAPI). Fetal bovine serum (FBS), trypsin-EDTA, Dulbecco’s modified Eagle’s medium (DMEM), and phosphate buffer saline solution (PBS) were bought from BI in Shanghai, China. Cell Counting Kit-8 (CCK-8), Calcein-AM/PI Double Staining Kit and Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit were outsourced from Do Jindo (Kumamoto, Japan). Coomassie brilliant blue was provided by Beyotime (Shanghai, China).