Adenosine triphosphate (ATP), L-methionine, L-cysteine, L-lysine, pyridoxal 5′-phosphate (PLP), adenylate kinase, phosphocreatine kinase, and phosphocreatine were purchased from Sigma. Uniformly labeled 13C and 15N L-lysine were purchased from Cambridge Isotope Laboratories. D,L-lysine-1,2-13C and D,L-lysine-2-13C were purchased from Aldrich. L-lysine-2,6,6-2H, L-lysine-3,3,4,4,5,5,6,6-2H and D,L-lysine-2-15N were purchased from CDN Isotopes. Sodium dithionite was purchased from Acros Organics and ammonium iron(II) sulfate was purchased from Fisher Chemicals. L-[Methyl-13C]-methionine was purchased from Cambridge Isotope Laboratories. All labeled substrates had a minimum of 99 atom % labeling. L-[U-14C] lysine was obtained from NEN Life Sciences Products. SAM and anSAM were prepared in our laboratory as described in the following sections. All other chemicals and reagents were of the highest purity and used as supplied.
Ammonium iron 2 sulfate
Manufactured by Thermo Fisher Scientific
Ammonium iron(II) sulfate is a chemical compound with the formula (NH4)2Fe(SO4)2·6H2O. It is a crystalline solid that is soluble in water. The compound serves as a source of iron(II) ions and ammonium ions for various applications in analytical chemistry and materials science.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine triphosphate (atp), by Merck Group (1 mentions)
L cysteine, by Merck Group (1 mentions)
L lysine, by Merck Group (1 mentions)
Sodium dithionite, by Thermo Fisher Scientific (1 mentions)
L methionine, by Merck Group (1 mentions)
Pyridoxal 5 phosphate, by Merck Group (1 mentions)
Phosphocreatine, by Merck Group (1 mentions)
Adenylate kinase, by Merck Group (1 mentions)
Phosphocreatine kinase, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Hplc grade methanol, by Merck Group (1 mentions)
Hplc grade ethanol, by Merck Group (1 mentions)
2 n morpholino ethanesulfonic acid mes, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Tris hcl, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Acetylcholine, by Merck Group (1 mentions)
Choline, by Merck Group (1 mentions)
2 protocols using ammonium iron 2 sulfate
1
Synthesis and Labeling of Lysine Derivatives
2
Histone H3K9 Peptide Analysis by MALDI-TOF
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Ascorbic acid, α-ketoglutarate, DMSO, 2-(N-morpholino) ethanesulfonic acid (MES), bovine serum albumin (BSA), acetylcholine, choline, Tris-HCl, trifluoroacetic acid (TFA), HPLC-grade water, HPLC-grade ethanol, HPLC-grade methanol, and HPLC-grade acetonitrile were purchased from Sigma-Aldrich (St. Louis, MO). Ammonium iron (II) sulfate and formic acid (FA) were purchased from Fisher Scientific (Hampton, NH). acetylcholinesterase was purchased from R&D Systems (Abingdon, UK). α-Cyano-4hydroxycinnamic acid matrix (CHCA), peptide calibration standard II, Anchor Chip PAC 384 HCCA plastic target plates, and metal MTP PAC Frames were purchased from Bruker Daltonics (Bremen, Germany). Histone H3K9 peptides were synthesized and HPLC purified to >95% purity by Cambridge Research Biochemicals (Cambridge, UK) as detailed by Hutchinson et al. 20
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