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Anti inos cxnft

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Anti-iNOS (CXNFT) is a specialized lab equipment designed for the detection and measurement of inducible nitric oxide synthase (iNOS) protein expression. It functions as a research tool for understanding nitric oxide signaling and regulation in various biological systems.

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Lab products found in correlation

Anti f4 80 clone bm8, by BioLegend (2 mentions) Anti cd11c hl3, by BD (2 mentions) Mr5d3, by OriGene (2 mentions) Anti tnf mp6 xt22, by BioLegend (2 mentions) Anti cd206, by BioLegend (2 mentions) Anti cd45 30 f11, by BD (2 mentions) Anti cd11b m1 70, by BD (2 mentions) Collagenase type 1, by Thermo Fisher Scientific (2 mentions) Facscanto 2 flow cytometer, by BD (2 mentions)

Spelling variants of this product

Anti-iNOS (20 citations) CXNFT (11 citations) INOS (CXNFT) (2 citations) PE anti-mouse iNOS (clone CXNFT, (2 citations)

2 protocols using anti inos cxnft

1

Isolation and Characterization of Stromal Vascular Fraction

Cited 1 time
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SVF was prepared as reported55 (link),59 (link),60 (link). Briefly, inguinal SAT or VAT were isolated from euthanized mice, minced and digested for 60 min in DMEM containing collagenase type I (2 mg/ml per gram of tissue; Invitrogen) at 37°C. Cell suspensions were then filtered through a 40-μm cell strainer and the SVF was collected as a pellet after centrifugation at 500g for 5 min. After RBC lysis, cells were stained with anti-CD45 (30-F11, BD), anti-F4/80 (clone BM8, BioLegend), anti-CD11b (M1/70, BD), anti-CD11c (HL3, BD), anti-CD206 (c06802, BioLegend; MR5D3, Acris), anti-TNF (MP6-XT22, BioLegend), anti-iNOS (CXNFT, eBioscience) and anti-CD49d [clone PS/2, AbD Serotec or clone 9C10(MFR4.B), BioLegend]. Cells were analyzed in a FACS Canto II flow cytometer (BD).
Chung K.J., Chatzigeorgiou A., Economopoulou M., Garcia-Martin R., Alexaki V.I., Mitroulis I., Nati M., Gebler J., Ziemssen T., Goelz S.E., Phieler J., Lim J.H., Karalis K.P., Papayannopoulou T., Blüher M., Hajishengallis G, & Chavakis T. (2017). A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity. Nature immunology, 18(6), 654-664.
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2

Isolation and Characterization of Stromal Vascular Fraction

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
SVF was prepared as reported55 (link),59 (link),60 (link). Briefly, inguinal SAT or VAT were isolated from euthanized mice, minced and digested for 60 min in DMEM containing collagenase type I (2 mg/ml per gram of tissue; Invitrogen) at 37°C. Cell suspensions were then filtered through a 40-μm cell strainer and the SVF was collected as a pellet after centrifugation at 500g for 5 min. After RBC lysis, cells were stained with anti-CD45 (30-F11, BD), anti-F4/80 (clone BM8, BioLegend), anti-CD11b (M1/70, BD), anti-CD11c (HL3, BD), anti-CD206 (c06802, BioLegend; MR5D3, Acris), anti-TNF (MP6-XT22, BioLegend), anti-iNOS (CXNFT, eBioscience) and anti-CD49d [clone PS/2, AbD Serotec or clone 9C10(MFR4.B), BioLegend]. Cells were analyzed in a FACS Canto II flow cytometer (BD).
Chung K.J., Chatzigeorgiou A., Economopoulou M., Garcia-Martin R., Alexaki V.I., Mitroulis I., Nati M., Gebler J., Ziemssen T., Goelz S.E., Phieler J., Lim J.H., Karalis K.P., Papayannopoulou T., Blüher M., Hajishengallis G, & Chavakis T. (2017). A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity. Nature immunology, 18(6), 654-664.
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