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Tcs nt upright confocal microscope

Manufactured by Leica camera
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The Leica TCS NT upright confocal microscope is a versatile research-grade instrument designed for high-resolution imaging. It utilizes confocal technology to capture detailed, optically sectioned images of samples. The instrument is capable of providing precise, three-dimensional information about the structure and distribution of fluorescently labeled specimens.

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Lab products found in correlation

Anti arrdc1, by Abcam (2 mentions) Anti mda5, by Abcam (2 mentions) Anti atg12, by Santa Cruz Biotechnology (2 mentions) Anti pink1, by Santa Cruz Biotechnology (2 mentions) Anti parkin mouse monoclonal clone prk8, by Santa Cruz Biotechnology (2 mentions) Anti miro, by Santa Cruz Biotechnology (2 mentions) Anti cd9 mm2 57, by Merck Group (2 mentions) Anti cd63 mem 259, by Thermo Fisher Scientific (2 mentions) Anti human mfge8, by Abnova (2 mentions) Anti dicer d11, by Santa Cruz Biotechnology (2 mentions) Anti tlr 7, by Cell Signaling Technology (2 mentions) Anti nf κb p50 alexa fluor 488 e 10, by Santa Cruz Biotechnology (2 mentions) Anti lc3, by Cell Signaling Technology (2 mentions) Anti gapdh, by Santa Cruz Biotechnology (2 mentions) Beta actin ac 15, by Merck Group (2 mentions) Anti tlr9, by Abcam (2 mentions) Anti lc3, by Abcam (2 mentions)

2 protocols using tcs nt upright confocal microscope

1

Western Blot Analysis of Autophagy Markers

Cited 1 time
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Western blot analysis was conducted as previously described44 (link). The following primary (at a concentration of 1.5 μg ml−1) and secondary (0.5 ng ml−1) antibodies were used for western blotting: polyclonal anti-ARRDC1, anti-LC3, anti-MDA5, anti-TLR9 (Abcam), polyclonal anti-Atg-12 (Santa Cruz Biotechnology, SC-68884), polyclonal anti-Pink1 (Santa Cruz Biotechnology, SC-33796), anti-Parkin mouse monoclonal (clone PRK8, Santa Cruz Biotechnology), polyclonal anti-Miro (Santa Cruz Biotechnology, SC-292547), anti-CD9 (MM2/57, Millipore), anti-CD63 (MEM-259, ThermoFisher), anti-TSG101 (51/TSG101, BD Biosciences), anti-Human MFGE8 (Abnova), anti-Dicer (D11, Santa Cruz Biotechnology, used at a concentration of 2 μg ml−1), anti-Tubulin, anti-LC3 (rabbit polyclonal), anti-TLR-7 (Cell Signaling Technology), Beta actin (AC-15, Sigma-Aldrich) and anti-GAPDH (Santa Cruz Biotechnology, SC-25778). NF-κB nuclear translocation was characterized by staining cells with anti NF-κB-P50 Alexa Fluor 488 (E-10, Santa Cruz Biotechnology). Images were obtained using a Leica TCS NT upright confocal microscope.
Phinney D.G., Di Giuseppe M., Njah J., Sala E., Shiva S., St Croix C.M., Stolz D.B., Watkins S.C., Di Y.P., Leikauf G.D., Kolls J., Riches D.W., Deiuliis G., Kaminski N., Boregowda S.V., McKenna D.H, & Ortiz L.A. (2015). Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nature Communications, 6, 8472.
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2

Western Blot Analysis of Autophagy and Inflammation Markers

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis was conducted as previously described 44 (link). The following primary (at a concentration of 1.5 μg/ml) and secondary (0.5 ng/ml) antibodies were used for Western Blotting: polyclonal anti-ARRDC1, anti-LC3, anti-MDA5, anti-TLR9 (Abcam), polyclonal anti-Atg-12 (Santa Cruz Biotechnology, SC-68884), polyclonal anti-Pink1 (Santa Cruz Biotechnology, SC-33796), anti-Parkin mouse monoclonal (clone PRK8, Santa Cruz Biotechnology), polyclonal anti-Miro (Santa Cruz Biotechnology, SC-292547), anti-CD9 (MM2/57, Millipore), anti-CD63 (MEM-259, ThermoFisher), anti-TSG101 (51/TSG101, BD Bioscciences), anti-Human MFGE8 (Abnova), anti-Dicer (D11, Santa Cruz Biotechnology, used at a concentration of 2 μg/ml), anti-Tubulin, anti-LC3 (rabbit polyclonal), anti-TLR-7 (Cell Signaling Technology), Beta actin (AC-15, Sigma Aldrich), anti-GAPDH (Santa Cruz Biotechnology, SC-25778). NFκB nuclear translocation was characterized by staining cells with anti NFκB -P50 Alexa Fluor 488 (E-10, Santa Cruz). Images were obtained using a Leica TCS NT upright confocal microscope.
Phinney D.G., Di Giuseppe M., Njah J., Sala E., Shiva S., St Croix C.M., Stolz D.B., Watkins S.C., Di Y.P., Leikauf G.D., Kolls J., Riches D.W., Deiuliis G., Kaminski N., Boregowda S.V., McKenna D.H, & Ortiz L.A. (2015). Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nature Communications, 6, 8472.
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