Dna ligase 3

Manufactured by Santa Cruz Biotechnology

DNA ligase 3 is an enzyme that catalyzes the formation of a phosphodiester bond between the 3' hydroxyl and 5' phosphate ends of double-stranded DNA fragments, facilitating the joining of DNA strands. It plays a crucial role in DNA repair and replication processes.

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Lab products found in correlation

Rad54, by Santa Cruz Biotechnology (2 mentions) Ap181p, by Merck Group (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Dna pkcs, by Fortis Life Sciences (2 mentions) Rad52, by Santa Cruz Biotechnology (2 mentions) Dnmt3a, by R&D Systems (1 mentions) Flag tag (flag), by Cell Signaling Technology (1 mentions) Rad51, by Santa Cruz Biotechnology (1 mentions) Gapdh antibody, by Cell Signaling Technology (1 mentions) Brca1, by Thermo Fisher Scientific (1 mentions) Brca2, by Santa Cruz Biotechnology (1 mentions) Slfn11, by Santa Cruz Biotechnology (1 mentions) Parp 1, by Santa Cruz Biotechnology (1 mentions) Rad51, by Abcam (1 mentions)

2 protocols using dna ligase 3

Comprehensive DNA Damage Response Protein Analysis

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Nuclear and total cell lysates were obtained as described before (29 (link)) and analyzed by SDS-PAGE using primary antibodies against: TET2 (Cell Signaling #45010), DNMT3A (R&D Systems, MAB63151 and Cell Signaling #2160), ATM (Santa Cruz Biotech #sc-135663), 53BP1 (Abcam #ab-21083), CtIP (ThermoFisher Scientific #PA5-20963), SLFN11 (Abcam #ab121731), BRCA1 (EMD Millipore #OP92-100UG), BRCA2 (Abcam #ab75335), PALB2 (ThermoFisher Scientific #PA5-20796), RAD51 (Santa Cruz Biotech #sc-8349), RAD52 (Santa Cruz Biotech #sc-365341), RAD54 (Santa Cruz Biotech #sc-374598), DNA-PKcs (Bethyl #A300-518A), DNA ligase 4 (Santa Cruz Biotech #sc-271299), Ku80 (ThermoFisher Scientific #MA5-15873), Ku70 (Santa Cruz Biotech #sc-17789), PARP1 (Santa Cruz Biotech #sc-74470), PARP2 (Abgent #AP12265a), PARP3 (Abgent #AP6296b), DNA ligase 3 (Santa Cruz Biotech #sc-135883), Flag (Cell Signaling #2368), lamin B (Abcam #ab-16048-100), and β-actin (Santa Cruz Biotech #sc-47778) and the following secondary antibodies conjugated to HRP: goat anti-rabbit (EMD Millipore #12-348) and goat anti-mouse (EMD Millipore #AP181P).

Maifrede S., Le B.V., Nieborowska-Skorska M., Golovine K., Sullivan-Reed K., Dunuwille W.M., Nacson J., Hulse M., Keith K., Madzo J., Caruso L.B., Gazze Z., Lian Z., Padella A., Chitrala K.N., Bartholdy B.A., Matlawska-Wasowska K., Marcantonio D.D., Simonetti G., Greiner G., Sykes S.M., Valent P., Paietta E.M., Tallman M.S., Fernandez H.F., Litzow M.R., Minden M.D., Huang J., Martinelli G., Vassiliou G.S., Tempera I., Piwocka K., Johnson N., Challen G.A, & Skorski T. (2021). TET2 and DNMT3A Mutations Exert Divergent Effects on DNA Repair and Sensitivity of Leukemia Cells to PARP Inhibitors. Cancer research, 81(19), 5089-5101.

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Analysis of DNA Damage Response Proteins

Cited 1 time

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Nuclear and total cell lysates were obtained as described before^{11 (link)} and analyzed by SDS-PAGE using primary antibodies against: ATM (Santa Cruz Biotechnology #sc-135663), CtIP (Abcam #ab-70163), 53BP1 (Abcam #ab-175933), SLFN11 (Santa Cruz Biotechnology #sc-515071), BRCA1 (ThermoFisher Scientific #MA1–23164), BRCA2 (Santa Cruz Biotechnology #sc-28235), PALB2 (Proteintech #14340–1-AP), RAD51 (Abcam #ab-88572), RAD52 (Santa Cruz Biotechnology #sc-365341), RAD54 (Santa Cruz Biotechnology #sc-374598), DNA-PKcs (Bethyl #A300–518A), Ku70 (Santa Cruz Biotechnology #sc-17789), Ku80 (ThermoFisher Scientific #MA5–15873), DNA ligase 4 (ThermoFisher Scientific #PA5–40826), PARP1 (Santa Cruz Biotechnology #sc-74470), PARP2 (Santa Cruz Biotechnology #sc-393310), PARP3 (Santa Cruz Biotechnology #sc-390771), DNA ligase 3 (Santa Cruz Biotechnology #sc-135883), Polθ (MyBioSource #MBS9612322), lamin B (Abcam #ab-16048–100), and β-actin (Santa Cruz Biotechnology #sc-47778) and the following secondary antibodies conjugated to HRP (horseradish peroxidase): goat anti-rabbit (EMD Millipore #12–348) and goat anti-mouse (EMD Millipore #AP181P). ZNF251 western analysis was performed with ZNF251 antibody (Proteintech cat# 25601–1-AP) and GAPDH antibody (Cell signaling technology cat#2118). For quantification of western analysis, ImageJ software was used to measure the density of the protein bands.

Li H., Chatla S., Liu X., Vekariya U., Kim D., Walt M., Lian Z., Morton G., Feng Z., Yang D., Liu H., Reed K., Childers W., Yu X., Madzo J., Chitrala K.N., Skorski T, & Huang J. (2023). Haploinsufficiency of ZNF251 causes DNA-PKcs-dependent resistance to PARP inhibitors in BRCA1-mutated cancer cells. Research Square.

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