Elvitegravir was provided by Gilead Sciences (Foster City, CA) via CONRAD (Arlington, VA). PLA-HPG was synthesized as previously described [25 (link)]. IR-780 iodide, glycerol, NaIO4, Na2SO3, bovine serum albumin (BSA), lactic acid, acetic acid, mucin, urea, glucose, Tween 80, Solutol (Kolliphor) HS 15, and HPLC-grade formic acid were obtained from Sigma-Aldrich. The 4-(4-(dihexadecylamino)styryl)-N-methylpyridinium iodide salt (DiA) and 4,6-diamidino-2 phenylindole (DAPI) stain were ordered from Invitrogen. Antibody stains anti-CD45 (ab10558) and anti-EpCAM (ab71916) and corresponding isotype controls for flow cytometry were purchased from Abcam. HPLC grade acetonitrile and water were purchased from VWR (J.T. Baker).
Anti epcam ab71916
Manufactured by Abcam
Sourced in United Kingdom
Anti-EpCAM (ab71916) is a recombinant antibody that recognizes the Epithelial Cell Adhesion Molecule (EpCAM). EpCAM is a transmembrane glycoprotein that is expressed on the surface of many epithelial cells and various types of cancer cells. This antibody can be used for the detection of EpCAM in various applications such as immunohistochemistry and western blotting.
Automatically generated - may contain errors
Lab products found in correlation
Na2so3, by Merck Group (1 mentions)
Naio4, by Merck Group (1 mentions)
Ir 780 iodide, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Avantor (1 mentions)
Hplc grade formic acid, by Merck Group (1 mentions)
Lactic acid, by Merck Group (1 mentions)
Mucin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Glycerol, by Merck Group (1 mentions)
Tween 80, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Solutol kolliphor hs 15, by Merck Group (1 mentions)
Anti cd45 ab10558, by Abcam (1 mentions)
Elvitegravir, by Gilead Sciences (1 mentions)
Trypsin, by Fujifilm (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Asp300, by Leica (1 mentions)
Anti vimentin 5741, by Cell Signaling Technology (1 mentions)
3 protocols using anti epcam ab71916
1
Elvitegravir Formulation and Characterization
2
Isolation of Gallbladder Epithelial Cells
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Gallbladder tissue was washed with ice-cold PBS at least five times. Any remaining mucus
gel was removed using forceps. To isolate epithelial cells, gallbladder samples were
digested with 0.25% w/v trypsin (Fujifilm Wako, Osaka, Japan) or 2,000 U/mL of dispase
(Goudou Shusei, Tokyo, Japan) at 37°C for 45 min. After inactivation of trypsin or dispase
by adding 10% fetal bovine serum, cells were collected by centrifugation at 1,500 rpm for
5 min. After another wash with PBS, the pelleted cells were stored at −80°C until
utilized. Cell viability was confirmed to be greater than 95% as assessed by the trypan
blue exclusion test. Additionally, the epithelial cells percentages assessed by
immunocytochemistry (anti-EpCAM, ab71916, Abcam, Cambridge, UK) in six of twenty samples
ranged from 92% to 98%.
gel was removed using forceps. To isolate epithelial cells, gallbladder samples were
digested with 0.25% w/v trypsin (Fujifilm Wako, Osaka, Japan) or 2,000 U/mL of dispase
(Goudou Shusei, Tokyo, Japan) at 37°C for 45 min. After inactivation of trypsin or dispase
by adding 10% fetal bovine serum, cells were collected by centrifugation at 1,500 rpm for
5 min. After another wash with PBS, the pelleted cells were stored at −80°C until
utilized. Cell viability was confirmed to be greater than 95% as assessed by the trypan
blue exclusion test. Additionally, the epithelial cells percentages assessed by
immunocytochemistry (anti-EpCAM, ab71916, Abcam, Cambridge, UK) in six of twenty samples
ranged from 92% to 98%.
3
Immunohistochemistry of Embryonic Tissues
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E10.5 embryos were fixed in 4% paraformaldehyde (PFA) at 4°C overnight and then orientated in 2% low-melting-point agarose in PBS. E17.5 embryo heads were fixed similarly then processed without agarose orientation. Samples were processed using a Leica ASP300S and embedded into paraffin. Embryo heads were sectioned in a coronal orientation, while E10.5 embryos were sectioned in a transverse or coronal orientation. Five-micrometre sections were cut onto Superfrost plus slides, and immunohistochemistry was performed using standard 3,3′-diaminobenzidine (DAB) protocols. The antibodies used were anti-E-cadherin (3195, batch 02/2017, Cell Signaling Technology; 1:100), anti-vimentin (5741S, batch 04/2017, Cell Signaling Technology; 1:100) and anti-Epcam (ab71916, batch GR231753-3, Abcam; 1:1200). Validation profiles for these antibodies are available on the supplier websites. H&E staining was performed using standard protocols. Immunohistochemical staining was quantified with ImageJ software using image deconvolution and a mask for DAB-positive areas.
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