An absorbable gelatin surgical sponge (Surgispon, Aegis Lifesciences, India) was cut manually with a blade into pieces of 9 mm3 (3 × 3 × 1 mm). The 1 × 106 cells were resuspended in 20 µl of the rat tail collagen, type I (Gibco, USA) (in the control group), and MgDCA or NaDCA salt (Sigma-Aldrich GmbH, Germany) dissolved in a medium (investigational medicine-treated groups). A 20 µl liquid mixture of tumor cells was pipetted onto a piece of a sponge. The NaDCA- or MgDCA-treated tumor groups and their controls were formed. At the EDD7, the tumor was grafted onto CAM among significant blood vessels. Its structural changes were observed with a stereomicroscope (SZX2-RFA16, Olympus, Japan) in vivo during the EDD9–12 period. The tumor images were acquired using a digital camera (DP92, Olympus, Japan) and CellSens Dimension 1.9 Digital Imaging Software.
Szx2 rfa16
Manufactured by Olympus
Sourced in Japan
The SZX2-RFA16 is a stereo zoom microscope with a built-in reflected fluorescence illuminator. It offers 6.3x to 100x total magnification range and a working distance of 30mm to 116mm. The microscope features high-performance optics, providing clear and detailed images for various applications.
Automatically generated - may contain errors
Lab products found in correlation
Cellsens dimension 1.9 digital imaging software, by Olympus (1 mentions)
Dp72 camera, by Olympus (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Rat tail collagen type 1, by Thermo Fisher Scientific (1 mentions)
Cellsens dimension software, by Olympus (1 mentions)
Bx40f4, by Olympus (1 mentions)
5 protocols using szx2 rfa16
1
In vivo Tumor Growth on Chick Chorioallantoic Membrane
2
In Vivo Chick Chorioallantoic Membrane Assay
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CAMs with grafted U87 cells were registered in vivo daily under a stereomicroscope (SZX2-RFA16, Japan) equipped with an Olympus DP72 camera for both video recordings and acquiring still images. CAMs were investigated from day 2 (EDD9) after grafting to day 5 (EDD12). Histological slides were investigated under a light microscope Olympus BX40F4 (Olympus Optical Co. Ltd., Japan) and photographed with an Olympus digital camera (XC30, Japan) using CellSens Dimension 1.9 Digital Imaging Software.
3
Temozolomide Effects on Tumor Grafts
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An absorbable gelatin surgical sponge (Surgispon, Aegis Lifesciences, India) was cut manually with a blade into pieces of 9 mm3 (3 × 3 × 1 mm). The 1 × 106 cells were resuspended in 20 µL of rat tail collagen, type I (Gibco, New York, NY, USA) (in the control group), and temozolomide (TMZ; Sigma Aldrich, St. Louis, MO, USA) dissolved in dimethyl sulfoxide (DMSO; Sigma Aldrich, St. Louis, MO, USA). A 20 µL liquid mixture of tumor cells was pipetted onto a piece of sponge. The 50 µM TMZ- and 100 µM TMZ-treated tumor groups and their controls were formed. At EDD7, the tumor was grafted onto the CAM among significant blood vessels. Its structural changes were observed with a stereomicroscope (SZX2-RFA16, Olympus, Tokyo, Japan) in vivo during the EDD9–12 period. The tumor images were acquired using a digital camera (DP92, Olympus, Tokyo, Japan) and CellSens Dimension 1.9 digital imaging software.
4
In Ovo Tumor Visualization
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Eggs with formed tumors were visualized in ovo daily from the second until the fifth day postgrafting (ninth day of embryo development [EDD9]-EDD12) under an Olympus stereomicroscope (SZX2-RFA16; Tokyo, Japan) equipped with an Olympus DP72 camera and photographed using CellSens Dimension 1.9 Digital Imaging Software.
5
In Ovo Chick Embryo Tumor Angiogenesis
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U87 cell tumors grafted on CAMs of chick embryos were observed in ovo daily and simultaneously from day 2 (EDD9) after tumor grafting to day 5 (EDD12) using an Olympus stereomicroscope SZX2-RFA16 (Olympus Corporation), supplied with an Olympus camera DP72 (Olympus Corporation, Tokyo, Japan) for acquiring both still images and video recordings. Examination of histological slides with H–E stained CAMs with tumors was performed using an Olympus light microscope BX40F4 (Olympus Corporation) equipped with an Olympus digital camera XC30 (Olympus Corporation). Recording the daily in ovo visualization of tumor on CAM and capture of H–E stained slides was performed by acquiring images with CellSens Dimension software (version 1.9, Olympus Corporation, Tokyo, Japan). The number of blood vessels in study groups was evaluated by photographing every H–E stained CAM at 4× magnification directly under the grafted tumor. All blood vessels bigger than 10 µm were counted in the CAM of the same length (1792 µm).
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