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3 protocols using fructo oligosaccharide

1

Gut Microbiome Modulation with Gracilaria Seaweed

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Gracilaria lemaneiformis was gained from the Nan’ao Island (Guangdong, China). Dextran sulfate sodium (DSS, 50 kDa) and the standards of six major SCFAs included acetate, butyrate, isobutyrate, propionate, isovalerate and valerate were provided by Aladdin Chemical Co. (Shanghai, China). Fructo-oligosaccharide was provided by Yuanye Biotechnology Co. (Shanghai, China). The primary antibodies, rabbit monoclonal CC-chemokine 9 receptor (CCR-9) and CC-chemokine ligand 25 (CCL-25), which are related to transport lymphocytes to intestine, were purchased from ABclonal, China. Moreover, we bought all of the secondary antibodies and primary antibodies for mouse monoclonal glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Beyotime, China. The reagents in the study were of analytical grade.
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2

Characterization of Carbohydrates Sourced

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Glucose (Glu), Fructose (Fru), Mannose (Man), Galactose (Gal), Xylose (Xyl), Arabinose (Ara), Rhamnose (Rha), Fucose (Fuc), Galactose Uronic acid (GalUA), glucuronic acid (GlcUA), purity ≥ 98%, β-dextran (purity ≥ 80%), fructo oligosaccharide (purity ≥ 95%), xylan (purity ≥ 98%) and polygalacturonic acid (purity ≥ 90%) were purchased from Yuanye Biotechnology Co., Ltd. D-trehalose (purity ≥ 99%) was purchased from Shanghai McLean Biochemical Technology Co., Ltd. All of other chemicals and reagents were analytical grade.
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3

Comprehensive Biomaterial Synthesis Protocol

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P407 and P188 were purchased from Kolliphor, BASF SE, Ludwigshafen, Germany. HA, lactose, glucomannan, lactulose alginate, and soluble starch were obtained from Shanghai Maclean Biochemical Technology Co., Ltd., Shanghai, China. Lactic acid, urea, acetic acid, and glucose were acquired from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Bovine serum albumin was purchased from Shanghai Huixing Biochemical Reagent Co., Ltd., Shanghai, China. Fructooligosaccharide (FOS) was provided by Shanghai Yuanye Bio-Technology Co., Ltd., Shanghai, China. Syto9 and propidium iodide (PI) were offered by KeyGEN Bio TECH, Nanjing, China.
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