Rabbit anti adamts5

Manufactured by Santa Cruz Biotechnology

Rabbit-anti-ADAMTS5 is a primary antibody that recognizes ADAMTS5, a disintegrin and metalloproteinase with thrombospondin motifs 5. ADAMTS5 is an enzyme involved in the degradation of aggrecan, a major component of the extracellular matrix in cartilage.

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Lab products found in correlation

Dmlb microscope, by Leica (3 mentions) Rabbit anti mmp13, by Abcam (3 mentions) Mouse anti active β catenin, by Merck Group (3 mentions) Mouse anti col2α1, by Santa Cruz Biotechnology (3 mentions) D3000 fluorescent microscope, by Leica (2 mentions) Rabbit anti dkk1, by Cell Signaling Technology (2 mentions) Mouse anti cbfβ, by Santa Cruz Biotechnology (2 mentions) Rabbit anti yap, by Santa Cruz Biotechnology (2 mentions) Rabbit anti sox9, by Santa Cruz Biotechnology (2 mentions) Goat anti rabbit igg fitc, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg fitc, by Santa Cruz Biotechnology (1 mentions) Rabbit anti runx1, by Abcam (1 mentions) Goat anti rabbit igg tritc, by Santa Cruz Biotechnology (1 mentions) P smad2 3, by Cell Signaling Technology (1 mentions) Rabbit anti yap, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Rabbit anti-TM (2 citations) Rabbit anti-mouse ADAMTS5 (2 citations)

3 protocols using rabbit anti adamts5

Immunohistochemistry and Immunofluorescence Assays

Cited 1 time

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Immunohistochemistry (IHC) and immunofluorescence (IF) were performed as previously described.^{10 (link)} The following primary antibodies were used: rabbit anti-Runx1 (Abcam, ab23980), mouse anti-Col2α1 (Santa Cruz, sc-52658), rabbit anti-MMP13 (Abcam, ab39012), rabbit anti-ADAMTS5 (Santa Cruz, sc-83186), rabbit anti-Yap (Cell Signaling Technology, 14074 S), p-Smad2/3 (Cell Signaling Technology, 8828 S), and mouse anti-active-β-catenin (Millipore, 05–665). The secondary antibodies were goat anti-rabbit IgG-FITC, goat anti-rabbit IgG-TRITC, goat anti-mouse IgG-FITC and goat anti-mouse IgG-TRITC from Santa Cruz. Images were taken by a Leica DMLB microscope and a Leica D3000 fluorescence microscope. ImageJ software was used to perform counts for the quantification of IHC or IF staining.

Zhang Y., Zuo T., McVicar A., Yang H.L., Li Y.P, & Chen W. (2022). Runx1 is a key regulator of articular cartilage homeostasis by orchestrating YAP, TGFβ, and Wnt signaling in articular cartilage formation and osteoarthritis. Bone Research, 10, 63.

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Immunofluorescence Assay of Cartilage Markers

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The following primary antibodies were used: mouse-anti-Cbfβ (Santa Cruz Biotechnology Cat# sc-56751, RRID:AB_781871), mouse-anti-Col2α1 (Santa Cruz Biotechnology Cat# sc-52658, RRID:AB_2082344), rabbit-anti-MMP13 (Abcam Cat# ab39012, RRID:AB_776416), rabbit-anti-ADAMTS5 (Santa Cruz Biotechnology Cat# sc-83186, RRID:AB_2242253), rabbit-anti-Sox9 (Santa Cruz Biotechnology Cat# sc-20095, RRID:AB_661282), rabbit-anti-Yap (Santa Cruz Biotechnology Cat# sc-15407, RRID:AB_2273277), rabbit-anti-Dkk1 (Cell Signaling Technology Cat# 48367, RRID:AB_2799337), and mouse-anti-Active-β-catenin(Millipore Cat# 05–665, RRID:AB_309887). Imaging was done with a Leica DMLB Microscope and a Leica D3000 fluorescent microscope and were quantified by Image J software.

Chen W., Lu Y., Zhang Y., Wu J., McVicar A., Chen Y., Zhu S., Zhu G., Lu Y., Zhang J., McConnell M, & Li Y.P. (2024). Cbfβ regulates Wnt/β-catenin, Hippo/Yap, and Tgfβ signaling pathways in articular cartilage homeostasis and protects from ACLT surgery-induced osteoarthritis. eLife, 13, e95640.

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Cartilage Extracellular Matrix Regulation

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The following primary antibodies were used: mouse-anti-Cbfβ (Santa Cruz Biotechnology Cat# sc-56751, RRID:AB_781871), mouse-anti-Col2α1 (Santa Cruz Biotechnology Cat# sc-52658, RRID:AB_2082344), rabbit-anti-MMP13 (Abcam Cat# ab39012, RRID:AB_776416), rabbit-anti-ADAMTS5 (Santa Cruz Biotechnology Cat# sc-83186, RRID:AB_2242253), rabbit-anti-Sox9 (Santa Cruz Biotechnology Cat# sc-20095, RRID:AB_661282), rabbit-anti-Yap (Santa Cruz Biotechnology Cat# sc-15407, RRID:AB_2273277), rabbit-anti-Dkk1 (Cell Signaling Technology Cat# 48367, RRID:AB_2799337), and mouse-anti-Active-β-catenin(Millipore Cat# 05–665, RRID:AB_309887). Imaging was done with a Leica DMLB Microscope and a Leica D3000 fluorescent microscope and were quantified by Image J software.

Chen W., Lu Y., Zhang Y., Wu J., McVicar A., Chen Y., Zhu S., Zhu G., Lu Y., Zhang J., McConnell M, & Li Y.P. (2024). Cbfβ regulates Wnt/β-catenin, Hippo/Yap, and TGFβ signaling pathways in articular cartilage homeostasis and protects from ACLT surgery-induced osteoarthritis. bioRxiv.

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