The total RNAs were extracted from cultured cells using TRIzol reagent (Takara Bio Inc., Dalian, China) according to a previously described method [27 (link)]. Six hundred ng of total RNA was reverse-transcribed into first-strand cDNA using the Prime RT Master Mix kit (Takara Bio Inc., Dalian, China). cDNA samples containing an equivalent amount of total RNA were quantified using SYBR Premix EX Taq II (Yeasen Biotech Co., Shanghai, China) with specific primer pairs and subjected to the CFX Connect™ Real-time PCR Detection System (Bio-Rad, Hercules, CA, USA). The specific primer pairs used are listed in Table S3 .
Prime rt master mix kit
Manufactured by Takara Bio
Sourced in China
The Prime RT Master Mix kit is a ready-to-use solution for reverse transcription of RNA to cDNA. The kit contains all the necessary components, including reverse transcriptase, an RNase inhibitor, and buffers, to efficiently convert RNA into complementary DNA.
Automatically generated - may contain errors
2 protocols using prime rt master mix kit
1
Extracting and Quantifying RNA from Cultured Cells
2
Quantitative RT-PCR Analysis of Gene Expression
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Total RNA was isolated from the cells or tissues using TRIzol reagent (Takara Bio Inc., China). Reverse transcription was performed according to the instruction of the Prime RT Master Mix kit (Takara Bio Inc., China). Quantitative real‐time polymerase chain reaction was performed in triplicate using SYBR Premix EX Taq II (Yeasen Biotech Co., Ltd. China). The sequences of primers were shown in Table S1 .
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