Forty eight hours after the last transfection, cells were imaged using an Olympus X81 microscope. All images were taken at a ×10 magnification. The image is representative of the well.
X81 microscope
Manufactured by Olympus
The Olympus X81 microscope is a high-performance optical microscope designed for various laboratory applications. It features a sturdy and ergonomic design, providing a stable platform for precise observations. The X81 microscope offers a range of magnification options to accommodate different sample types and analysis requirements.
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5 protocols using x81 microscope
1
Fluorescence Microscopy Imaging Protocol
2
Fluorescent Imaging of Silver Nanoparticle-Nucleobase Interactions
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Corresponding C12-stabilized silver nanoparticles and nucleobases were mixed in DI water. 20 μL of the above solution was dropped onto a glass coverslip and left in the dark at room temperature. It was then imaged under an Olympus X81 microscope before or after being fully dewetted. Samples were excited with 100 W mercury lamp filtered by a 545–580 nm band pass filter and monitored after filtered by a 610 nm long pass filter (green excitation). The filter sets for adenine (UV excitation) were BP 360–370 for excitation and BP 420–460 for emission. For SEM and TEM imaging, samples were dropped on TEM grids.
3
Immunofluorescence Analysis of p65 Localization
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Cells were seeded into 24-well plates that were pre-inserted with glass slides. After receiving indicated treatment, the cells were fixed with 4% paraformaldehyde for 15 min, followed by permeabilization with 0.2% Triton X-100 solution for 5 min. Next, the cells were blocked with normal goat serum for 30 min, incubated with p65 primary antibodies (1:100) overnight, and Goat anti-mouse IgG Alexa Fluor-594 secondary antibody (1:200) for 1 h. DAPI was used to indicate the nucleus. The slides were covered with antifade mounting medium and coverslips. Images were captured with an X81 microscope (Olympus).
4
Quantification of ER and PR Expression in Breast Cancer Cell Lines
5
Immunofluorescence Staining of Cultured Cells
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Cells seeded on glass slides were fixed with 4% paraformaldehyde for 15 min at RT, permeabilized with 0.2% Triton X-100 for 5 min at RT, and blocked with normal goat serum for 30 min at RT before being incubated with appropriate primary antibodies (1:100) overnight at 4 °C and corresponding secondary antibody (1:200) for 1 h at 37 °C. The nuclei were counterstained with DAPI for 5 min at RT. The slides were covered with antifade mounting medium and coverslips. Images were collected using an X81 microscope (Olympus).
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