Serum samples collected during COH were assayed for FSH, LH, E2, and P4 using the Roche Electrical Chemiluminescence Immunoassay (Roche, Basel, Switzerland) and all the 480 samples were tested. Serum SHBG concentrations were assayed using commercially available enzyme linked immunosorbent assay kits (R&D systems, MN, USA). All assays were carried out according to standard protocols by the same experienced technician to minimize the effect of interassay variability.
Electrical chemiluminescence immunoassay
Manufactured by Roche
Sourced in Switzerland
The Roche electrical chemiluminescence immunoassay is a laboratory equipment designed for the quantitative determination of analytes in biological samples. It utilizes the principle of chemiluminescence to detect and measure specific proteins, hormones, or other molecules of interest.
Automatically generated - may contain errors
3 protocols using electrical chemiluminescence immunoassay
1
Hormonal Assays in Serum Samples
2
Estradiol Quantification in Cell Culture and Rat Serum
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KGN cells (2 × 105) were seeded onto six-well plates. After siRNA treatment for 48 h or vector treatment for 72 h, we changed fresh culture medium containing certified charcoal stripped fetal bovine serum (Gibco, Grand Island, NY). Then testosterone (T) (10−7 mol/l) was added into cells and the culture medium was collected 3 h later. The culture medium was centrifuged at a speed of 12000 rpm/min for 5 min before the concentration of E2 was detected using Roche electrical chemiluminescence immunoassay after 40 times dilution.
The E2 concentration of rat serum before hCG administration was detected using Estradiol ELISA Kit (Cayman Chemical, Ann Arbor, MI).
The E2 concentration of rat serum before hCG administration was detected using Estradiol ELISA Kit (Cayman Chemical, Ann Arbor, MI).
3
Perioperative Hemodynamic and ACTH Dynamics
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The systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), and HR were collected at baseline and then again at 1-minute intervals for 10 minutes after skin incision. Plasma ACTH concentrations were measured using the Roche electrical chemiluminescence immunoassay at baseline and 10 minutes after skin incision. The selection of the data collection timepoint was based on the study by Donald et al. (10 (link)), which demonstrated that in a conventional cholecystectomy, ACTH levels peak at 10 minutes after incision. Hemodynamic variables were also collected before the surgery. Other baseline patient characteristics, including age, body mass index (BMI), liver function, and renal function, were also collected.
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