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Rnase staining solution

Manufactured by Merck Group
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RNase staining solution is a laboratory reagent used to detect the presence of RNase enzymes. It contains a dye that binds to and stains RNA-degrading RNase enzymes, allowing their visualization and localization within biological samples.

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Close spelling variants of this product

RNase solution (18 citations) RNases (8 citations) PI/RNase staining solution (3 citations)

2 protocols using rnase staining solution

1

Cell Cycle Analysis by Flow Cytometry

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Cell cycle assay was performed by flow cytometry to analyze the cell cycle progression while treating with Ps-GOS for 48 h. Cells were trypsinized and fixed in 70% ethanol at 4 °C for 30 min, followed by incubation with propidium iodide containing RNase staining solution (Merck, Branchburg, NJ, USA) for 30 min in the dark to detect DNA content in the cells. The percentage of cells in G0/G1, S, and G2/M phases was analyzed by flow cytometry Guava easyCyteTM HT (Hayward, CA, USA).
Yodthong T., Kedjarune-Leggat U., Smythe C., Sukprasirt P., Aroonkesorn A., Wititsuwannakul R, & Pitakpornpreecha T. (2020). Enhancing Activity of Pleurotus sajor-caju (Fr.) Sing β-1,3-Glucanoligosaccharide (Ps-GOS) on Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells through the Involvement of BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway. Biomolecules, 10(2), 190.
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2

Cell Cycle Analysis by Flow Cytometry

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Cells treated with l-quebrachitol for 48 h were trypsinized, fixed in ice-cold 70% ethanol at 4 °C at least 30 min, and washed with PBS after fixing. Then, propidium iodide containing RNase staining solution (Merck, New Jersey, USA) was added to determine the DNA content and incubated for 30 min at room temperature in the dark. Each sample (1 × 106 cells) was analyzed by flow cytometry (Guava easyCyteTM HT, Hayward, CA, USA) to determine the percentages of G1, S, and G2 in the cell cycle.
Yodthong T., Kedjarune-Leggat U., Smythe C., Wititsuwannakul R, & Pitakpornpreecha T. (2018). l-Quebrachitol Promotes the Proliferation, Differentiation, and Mineralization of MC3T3-E1 Cells: Involvement of the BMP-2/Runx2/MAPK/Wnt/β-Catenin Signaling Pathway. Molecules, 23(12), 3086.
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