Rat il 6 elisa kit

Anti-CaSR-Ab was purchased from NOVUS (Colorado, USA). Anti-P-65-Ab and anti-P-p65-Ab were from Cell Signaling Technology (Boston, USA). Histopaque1083, Histopaque1119, and fMLP were from Sigma (Darmstadt, Germany). NPS-2143, cincalcet, and PDTC were purchased from MCE (New Jersey, USA). Rat IL-6 ELISA kit was from Elabscience (Wuhan, China), rat MPO ELISA kit was from USCN (Wuhan, China), and rat IL-10 was from CUSABIO (Wuhan, China). DHE-ROS kit was from BestBio (Shanghai, China), and NO assay kit was from Beyotime (Shanghai, China).

Biological samples were collected from all the subjects in all three phases of the experiment. Blood was collected through retro-orbital sinus puncture, using a capillary glass tube. Approximately 1 mL of blood was harvested in sterile vacutainers laced with 3.2% sodium citrate, to prevent coagulation. Immediately after, the vacutainers were centrifuged for 10 min, at 400 rpm, to obtain plasma samples. The processed samples were stored in sterile Eppendorf containers at −80 °C. The ELISA technique was employed to assess plasma levels of IL-1, IL-6 and TNF-α, using commercially available kits: Rat IL-1β ELISA Kit, Elabscience^® Biotechnology Inc, Wuhan, Hubei, China, Rat IL-6 ELISA Kit, Elabscience^® Biotechnology Inc, Wuhan, Hubei, China, and Rat TNF-α ELISA Kit, Elabscience^® Biotechnology Inc, Wuhan, Hubei, China.
Stimulated saliva was harvested under anaesthesia, using a citric acid solution, obtained by dissolving 8 g of citric acid in 20 mL sterile 0.9% sodium chlorite solution. After wiping the excessive citric acid solution from the oral cavity with a sterile gauze, approximately 2 mL of saliva was harvested in sterile Eppendorf containers and stored at −80 °C. The salivary levels of MMP-8 were assessed in all the three phases of the experiment using the commercially available ELISA kit: Rat MMP-8 ELISA Kit, RayBio^®, Norcross, GA, USA.