Anti phgdh antibody

Manufactured by Merck Group

Sourced in United States

The Anti-PHGDH antibody is a laboratory reagent used to detect and quantify the expression of the PHGDH protein in biological samples. PHGDH is an enzyme involved in serine biosynthesis. The antibody can be used in various techniques, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the role of PHGDH in cellular processes.

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Lab products found in correlation

Protease inhibitor complex, by Roche (1 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions) Ripa buffer, by Avantor (1 mentions) Western lighting plus ecl, by PerkinElmer (1 mentions) Anti β actin antibody, by Cell Signaling Technology (1 mentions) Chemidoc touch imaging system, by Bio-Rad (1 mentions) Ab1801, by Abcam (1 mentions) Anti actin antibody, by Abcam (1 mentions) Hpa021241, by Abcam (1 mentions)

2 protocols using anti phgdh antibody

Western Blot Protein Detection Protocol

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Cells were lysed in RIPA buffer (VWR International) containing freshly added protease inhibitor complex (Roche). 20 μg of protein was loaded on precast NuPAGE Bis-Tris gels (Thermo Fisher Scientific) followed by transfer onto nitrocellulose. Chemiluminescent signals were detected with Western Lighting Plus-ECL (Perkin Elmer, NEL103001) and imaged with the ChemiDoc Touch Imaging System (Bio-Rad). Anti-PHGDH antibody was purchased from Sigma (WH0026227M1), and was used at 1:1000 dilution. Anti-β-ACTIN antibody was purchased from Cell Signaling (3700S), and was used at 1:2000 dilution. Uncropped western blot images are reported in Supplementary Figure 13.

Reid M.A., Allen A.E., Liu S., Liberti M.V., Liu P., Liu X., Dai Z., Gao X., Wang Q., Liu Y., Lai L, & Locasale J.W. (2018). Serine synthesis through PHGDH coordinates nucleotide levels by maintaining central carbon metabolism. Nature Communications, 9, 5442.

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Immunoblotting Protocol for PHGDH and Actin

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PHGDH protein was detected using a rabbit polyclonal anti-PHGDH antibody from Sigma, St. Louis, MO, USA (HPA021241) at 0.2 μg/ml and actin detected using a rabbit polyclonal anti-actin antibody from Abcam, Cambridge, MA, USA (ab1801) at 1 μg/ml, appropriate HRP-conjugated secondary antibodies and chemiluminescence.

Mattaini K.R., Brignole E.J., Kini M., Davidson S.M., Fiske B.P., Drennan C.L, & Vander Heiden M.G. (2015). An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation. Cancer & Metabolism, 3, 5.

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