Pdsred monomer f

Manufactured by Takara Bio

PDsRed monomer-F is a monomeric red fluorescent protein derived from the sea anemone Discosoma sp. It has an excitation maximum at 556 nm and an emission maximum at 586 nm.

Automatically generated - may contain errors

Lab products found in correlation

Pegfp f, by Takara Bio (4 mentions) Superfect transfection reagent, by Qiagen (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Merck Group (3 mentions) Viafect, by Promega (3 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Horse serum, by Thermo Fisher Scientific (2 mentions) Lsm 710 microscope, by Zeiss (2 mentions) Rat anti ha antibody 1 5000, by Roche (2 mentions) Streptomycin, by Merck Group (1 mentions) Penicillin, by Merck Group (1 mentions) Dmem f12, by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Poly l lysine solution, by Merck Group (1 mentions) Glass coverslip, by Matsunami (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Amphotericin b, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Merck Group (1 mentions) Usinglipofectamine 2000, by Thermo Fisher Scientific (1 mentions)

6 protocols using pdsred monomer f

Transient Transfection of HEK293 and PC12 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 100 unit ml^–1 penicillin and 100 µg ml^–1 streptomycin and 10% FBS (Sigma) at 37 °C in a humidified atmosphere of 95% air and 5% CO₂. HEK293 cells were transfected with plasmids (WT mGlu1, the mGlu1 mutants, or the control vector) using SuperFect transfection reagent (Qiagen) or Viafect (Promega) according to the manufacturer’s instruction. The cells were co-transfected with pEGFP-F (Clontech), pDsRed monomer-F (Clontech), or piRFP670-N1 (ref. ^{62 (link)}) as the transfection marker. After transfection, the cells were cultured in DMEM supplemented with 10% dialyzed FBS (Gibco) instead of 10% FBS to decrease cytotoxicty. After 36–48 h incubation, the transfected HEK293 cells were utilized for experiments. piRFP670-N1 is a gift from Vladislav Verkhusha (Addgene plasmid # 45457).
PC12 cells were maintained in DMEM supplemented with 100 unit ml^–1 penicillin and 100 µg ml^–1 streptomycin and 10% horse serum (Gibco) at 37 °C in a humidified atmosphere of 95% air and 5% CO₂.

Ojima K., Kakegawa W., Yamasaki T., Miura Y., Itoh M., Michibata Y., Kubota R., Doura T., Miura E., Nonaka H., Mizuno S., Takahashi S., Yuzaki M., Hamachi I, & Kiyonaka S. (2022). Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue. Nature Communications, 13, 3167.

+ Open protocol

+ Expand

Trpc6-HA M131T Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the ability of pCDNA3 Trpc6-HAM131T to express the proteins we performed Western blot and immunofluorescence analysis as described before [21 ]. Briefly, for Western blot HEK293 cells were transfected with the plasmid containing the Trpc6-HA M131T cDNA and lysed 16 hours after transfection. Expression was confirmed with rat anti-HA antibody 1/5000 (clone 3F10, Roche). To determine subcellular localization, HeLa cells were co-transfected with the same construct and method. Plasmid pDsRed Monomer-F (Clontech) was also transfected for membrane co-localization analysis. The same rat anti-HA antibody(1/500) was used for detecting Trpc6 M131T membrane signal. Mounted slides were analyzed with confocal Zeiss LSM710 microscope and its respective software.

Canales C.P., Krall P., Kairath P., Perez I.C., Fragoso M.A., Carmona-Mora P., Ruiz P., Reiser J., Young J.I, & Walz K. (2014). Characterization of a Trpc6 Transgenic Mouse Associated with Early Onset FSGS. British journal of medicine and medical research, 5(10), 1198-2012.

+ Open protocol

+ Expand

Culturing and Transfecting Cell Lines for Receptor Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293T, HEK293, and CHO
cells were maintained in Dulbecco’s
modified Eagle’s medium (DMEM, Sigma-Aldrich) for HEK293T and
HEK293 cells or DMEM-F12 (Sigma-Aldrich) for CHO cells supplemented
with 10% fetal bovine serum (FBS) (Gibco), 100 unit/mL penicillin,
100 μg/mL streptomycin, and 0.25 μg/mL amphotericin B
(Gibco) at 37 °C in a humidified atmosphere of 95% air and 5%
CO₂. For β₂ARs, HEK293T and HEK293 cells
were transiently transfected with plasmids (WT β₂ARs, the β₂AR mutants, or the control vector) using
Lipofectamine2000 (Invitrogen) and Superfect transfection reagent
(Qiagen), respectively, in DMEM supplemented with 10% FBS according
to the manufacture’s instruction. For M1R, CHO cells were transiently
transfected with plasmids (WT M1R, the M1R mutants, or the control
vector) using Lipofectamine2000 transfection reagent in DMEM-F12 supplemented
with 10% FBS according to the manufacture’s instruction. The
cells were co-transfected with pEGFP-F (Clontech), pmCherry-F,^{36 (link)} or pDsRed monomer-F (Clontech) as a transfection
marker. For Ca²⁺ imaging, the cells were grown for 24–36
h, seeded on glass coverslips (Matsunami) coated with poly-l-lysine solution (Sigma-Aldrich), and subjected to Ca²⁺ imaging 4–10 h after seeding.

Kubota R., Nomura W., Iwasaka T., Ojima K., Kiyonaka S, & Hamachi I. (2018). Chemogenetic Approach Using Ni(II) Complex–Agonist Conjugates Allows Selective Activation of Class A G-Protein-Coupled Receptors. ACS Central Science, 4(9), 1211-1221.

+ Open protocol

+ Expand

Transient Transfection of HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) with 100 unit ml⁻¹ penicillin and 100 μg ml⁻¹ streptomycin and 10% FBS (Sigma) at 37°C in a humidified atmosphere of 95% air and 5% CO₂. HEK293 cells were transiently transfected with plasmids encoding WT mGlu 1, the mGlu1 mutants, or the control vector using Viafect (Promega) following the manufacture’s instruction. The cells were co-transfected with pEGFP-F (Clontech), pDsRed monomer-F (Clontech), or iRFP-670 (kindly gifted from Prof. Verkhusha) as transfection markers. For the culture of transfected cells, DMEM supplemented with 10% dialyzed FBS (Sigma) was used to decrease the cytotoxicity. The medium was exchanged 4 h after the transfection, and the cells were used for the experiments after 24–48 h.

Senoo A., Yamada Y., Ojima K., Doura T., Hamachi I, & Kiyonaka S. (2022). Orthogonal Activation of Metabotropic Glutamate Receptor Using Coordination Chemogenetics. Frontiers in Chemistry, 9, 825669.

+ Open protocol

+ Expand

Trpc6-HA M131T Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Transfection and Culture of HEK293 and PC12 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 100 unit ml -1 penicillin and 100 µg ml -1 streptomycin and 10% FBS (Sigma) at 37 °C in a humidified atmosphere of 95% air and 5% CO2. HEK293 cells were transfected with plasmids (WT mGlu1, the mGlu1 mutants, or the control vector) using SuperFect transfection reagent (Qiagen) or Viafect (promega) according to the manufacturer's instruction. The cells were co-transfected with pEGFP-F (Clontech), pDsRed monomer-F (Clontech), or iRFP670 (kindly gifted from Prof. Verkhusha) as the transfection marker. After transfection, the cells were cultured in DMEM supplemented with 10% dialyzed FBS (Gibco) instead of 10% FBS to decrease cytotoxicty. After 36-48 h incubation, the transfected HEK293 cells were utilized for experiments. PC12 cells were maintained in DMEM supplemented with 100 unit ml -1 penicillin and 100 µg ml -1 streptomycin and 10% horse serum (Gibco) at 37 °C in a humidified atmosphere of 95% air and 5% CO2.

Ojima K., Kakegawa W., Itô M., Miura Y., Michibata Y., Kubota R., Doura T., Yamasaki T., Miura E., Nonaka H., Mizuno S., Takahashi S., Yuzaki M., Hamachi I., & Kiyonaka S. (2021). Coordination chemogenetics for activation of GPCR-type glutamate receptors in brain tissue.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!