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Anti gr 1 apc rb6 8c5

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Anti-GR-1-APC (RB6-8C5) is a fluorochrome-conjugated antibody that binds to the GR-1 antigen. GR-1 is a cell surface marker expressed on myeloid-derived suppressor cells (MDSCs) and neutrophils. This antibody can be used to identify and quantify these cell populations in flow cytometry applications.

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Lab products found in correlation

Facsaria 2 flow cytometer, by BD (1 mentions) Fitc anti cd11b m1 70, by BioLegend (1 mentions) Anti ifn γ apc xmg1.2, by BD (1 mentions) Facscalibur cytometer, by BD (1 mentions) Lps from salmonella typhimurium, by Merck Group (1 mentions) Adenosine deaminase ada, by Roche (1 mentions) 5 amp, by Merck Group (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Pe cy7 anti sca 1 d7, by Thermo Fisher Scientific (1 mentions) Percp cy5, by BioLegend (1 mentions) Biotin anti lineage cocktail, by Thermo Fisher Scientific (1 mentions) Percp cy5.5 streptavidin, by Thermo Fisher Scientific (1 mentions) Apc anti c kit 2b8, by Thermo Fisher Scientific (1 mentions) Edta microtainers, by BD (1 mentions) Brilliant violet 421, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

RB6-8C5 (41 citations) Gr-1 (RB6-8C5, (23 citations) Gr-1-APC (16 citations) Anti-Gr-1 (RB6-8C5) (10 citations) Anti-Gr-1-APC (9 citations) Anti Gr-1-APC (clone RB6-8C5, (2 citations)

4 protocols using anti gr 1 apc rb6 8c5

1

Characterizing T Helper Cell and Neutrophil Populations

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Cellular staining and flow cytometry analyses for T helper cells and neutrophils were conducted as previously described [9 (link)]. Briefly, following stimulation with PMA and ionomycin, NALT cells were treated with Brafeldin A and stained for surface markers with anti-CD4-FITC (GK1.5, BD Biosciences) for T helper cells and with anti- CD11b-FITC (M1/70, Biolegend) and anti- GR-1-APC (RB6-8C5, Biolegend) for neutrophils. For intracellular staining, fixed cells were permeabilized and stained with anti-IL-17A-PE (TC11-18H10, BD Biosciences) for Th17, anti-IFN-γ-APC (XMG1.2, BD Biosciences) for Th1 cells and anti-IL-4-PerCP-eFluo®710 (11B11, eBioscience) for Th2 cells. Samples were analyzed on a FACSAriaII flow cytometer (BD Biosciences) using FlowJo software (Tree Star).
Chen X., Li N., Bi S., Wang X, & Wang B. (2016). Co-Activation of Th17 and Antibody Responses Provides Efficient Protection against Mucosal Infection by Group A Streptococcus. PLoS ONE, 11(12), e0168861.
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2

Quantification of Immune Cell Subsets in BALF

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Broncheoalveolar lavage fluids (BALFs) was collected by instillation of 0.8 ml of PBS through a tracheal cannula. BALF samples were centrifuged at 1500 rpm for 5 min at 4°C. Supernatants were collected and stored for cytokine measurements. The number of total cells, eosinophils, neutrophils, lymphocytes and macrophages was determined by flow cytometry as previously described (32 (link)). Briefly, cells were blocked with CD16/32 antibody for 15 min, then incubated for 30 min with antibodies as following: anti-Siglet F-PE (S17007L, BioLegend), anti-Mac-3- FITC (M3/84, BioLegend), anti-Gr-1- APC (RB6-8C5, BioLegend), anti-CD3ϵ PerCP-Cyanine5.5 (145-2C11, BioLegend). Cells were analyzed on a FACSCalibur cytometer (BD Biosystems). SSChighSiglec-F+Mac-3- and SSChighSiglec-F+Mac-3+ cells were identified as eosinophils and alveolar macrophages, respectively. FSChighSSChigh Gr-1+cells were recognized as neutrophils. FSClowSSClowCD3+ cells were identified as lymphocytes.
Tu W., Xiao X., Lu J., Liu X., Wang E., Yuan R., Wan R., Shen Y., Xu D., Yang P., Gong M., Gao P, & Huang S.K. (2023). Vanadium exposure exacerbates allergic airway inflammation and remodeling through triggering reactive oxidative stress. Frontiers in Immunology, 13, 1099509.
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3

Multi-color Flow Cytometric Analysis of Immune Cells

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Anti-CD3ε (145-2C11), anti-CD16/CD32 (2.4G2), PE-anti-CD73 (TY23), APC-anti-CD39 (TÜ66), FITC-anti-CD21/35 (7G6), PE- and APC-anti-PD-L2 (TY25), and FITC-anti-IgMa (DS-1) were obtained from BD Biosciences (San Diego, CA, USA). Alexa Flour 647-anti-CD73 (TY11.8), FITC- and perCP-Cy5.5-anti-B220 (RA3-6B2), perCP-Cy5.5-F4/80, Alexa Fluor 647-anti-CD5 (53-7.3), APC-anti-CD93 (AA4.1), and APC-anti-Gr-1 (RB6-8C5) were obtained from Biolegend. PE-Cy7-anti-CD23 (2G8) was obtained from Abcam. PE-anti-IL-10 (JES5–16E3) was obtained from eBioscience (San Diego, CA). Anti-CD40 (1C10) was obtained from R&D Systems. Affinity-purified F(ab’)2 fragments of goat anti-mouse IgM (anti-Ig) were obtained from Jackson Immunoresearch Laboratories. LPS from Salmonella typhimurium, and 5′-AMP, were obtained from Sigma Aldrich. Adenosine Deaminase (ADA) was obtained from Roche Diagnostics (Indianapolis, IN).
Kaku H., Cheng K.F., Al-Abed Y, & Rothstein T.L. (2014). A novel mechanism of B-cell mediated immune suppression through CD73-expression and adenosine production. Journal of immunology (Baltimore, Md. : 1950), 193(12), 5904-5913.
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4

Comprehensive Blood Cell Phenotyping

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Antibodies were from Pharmingen unless otherwise specified. Peripheral blood was obtained by lancing the facial vein and collecting several drops into EDTA microtainers (Pharmingen). Blood counts were obtained using a Hemavet 50FS. Blood elements were enumerated using PE or PerCP-Cy5.5-anti-CD3 (145-2C11), PE-anti-B220 (RA3-6B2), APC-anti-CD19 (1D3), APC, PE or PerCP-Cy5.5-anti-CD11b (M1/70), PE or PerCP-Cy5.5-anti-Ter119 (Ter119), and PerCP-Cy5.5 or APC-anti-Gr-1 (RB6-8C5, Biolegend). Stem and progenitor cells were enumerated using biotin-anti-Lineage Cocktail, PerCP-Cy5.5-streptavidin, APC-anti-c-Kit (2B8) and PE-Cy7-anti-Sca-1 (D7, eBioscience), in addition to PE-anti-CD16/CD32 (FcγR, 2.4G2) and Brilliant Violet 421-anti-CD34 (RAM34) for CMP, GMP, and MEP, PE-Texas Red-anti-CD127 (IL7R, SB199) for CLP, or Brilliant Violet 421-anti-CD34 and PE-anti-CD135 (FLT3, A2F10.1) for MPP, ST-HSC, and LT-HSC. Alternatively, LSK cells were stained using PE—anti-CD150 (Q38-480) and Brilliant Violet 421–anti-CD48 (HM48-1) for LSK/SLAM LT-HSC. Marrow subsets for RNA analysis were obtained after lineage-depletion and antibody staining via a FACSAria II cell sorter (BD Biosciences).
Guo H., Cooper S, & Friedman A.D. (2016). In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis. PLoS ONE, 11(3), e0150809.
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