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Anti igg

Manufactured by ABclonal
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Sourced in China

Anti-IgG is a laboratory reagent used to detect and quantify immunoglobulin G (IgG) in biological samples. It functions by specifically binding to IgG, allowing for its identification and measurement.

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Lab products found in correlation

Anti ago2, by ABclonal (4 mentions) Anti eif4a3, by Proteintech (2 mentions) Magna rip rna binding protein immunoprecipitation kit, by Merck Group (1 mentions) Rna immunoprecipitation kit, by BersinBio (1 mentions) Anti m6a, by Abcam (1 mentions) Rip assay kit, by BersinBio (1 mentions) Rna binding protein immunoprecipitation kit, by BersinBio (1 mentions)

6 protocols using anti igg

1

RIP Assay for circIGF1R and miR-16

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Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Boston, MA, USA) was used for RIP assay. The antibodies included anti-AGO2 (Boster, Wuhan, China) and anti-IgG (ABclonal, Wuhan, China). The beads were then incubated with cell lysate to immune-precipitate the RNAs bound to the beads. Finally, the levels of circIGF1R and miR-16 were detected using qRT-PCR.
Li M., Zhang N., Li J., Ji M., Zhao T., An J., Cai C., Yang Y., Gao P., Cao G., Guo X, & Li B. (2023). CircRNA Profiling of Skeletal Muscle in Two Pig Breeds Reveals CircIGF1R Regulates Myoblast Differentiation via miR-16. International Journal of Molecular Sciences, 24(4), 3779.
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2

RIP Assay with BersinBio Kit

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BersinBioTM RNA Immunoprecipitation Kit (BersinBio, China) was used for conducting RIP assay according to the manufacturer’s instructions. Anti-Ago2 (Abclonal, China), anti-EIF4A3 (Proteintech, China) or anti-IgG (Abclonal, China) were utilized for immunoprecipitation. The enriched RNAs were further analyzed by qRT-PCR.
Wang X., Song H., Fang L, & Wu T. (2022). EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway. Cell Death Discovery, 8, 92.
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3

RNA Immunoprecipitation and Methylated RNA Analysis

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RIP and MeRIP was conducted using the BersinBioTM RNA Immunoprecipitation Kit (Guangzhou, China) and BersinBioTM Methylated RNA immunoprecipitation according to manufacturer’s instructions, respectively. Anti-AGO2 (Abclonal, China), Anti-m6A (Abcam, USA) and anti-IgG (Abclonal, China) were used. The extracted RNAs were analyzed by qRT-PCR.
Wang X., Jian W., Luo Q, & Fang L. (2022). CircSEMA4B inhibits the progression of breast cancer by encoding a novel protein SEMA4B-211aa and regulating AKT phosphorylation. Cell Death & Disease, 13(9), 794.
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4

AGO2-Mediated RNA Immunoprecipitation in MDA-MB-231

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RIP assay was conducted in MDA-MB-231 cells using the BersinBioTM RNA-Binding Protein Immunoprecipitation Kit (Guangzhou, China) according to manufacturer’s instructions. Anti-AGO2(Abclonal, China) and anti-IgG(Abclonal, China) were used. The extracted RNAs were analyzed by RT-qPCR.
Wang X., Ji C., Hu J., Deng X., Zheng W., Yu Y., Hua K., Zhou X, & Fang L. (2021). Hsa_circ_0005273 facilitates breast cancer tumorigenesis by regulating YAP1-hippo signaling pathway. Journal of Experimental & Clinical Cancer Research : CR, 40, 29.
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5

RIP Assay for EIF4A3 Interactions

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A BersinBio RIP assay kit (BersinBio, Guangzhou, China) was used for RIP assays. Cell lysates were incubated with magnetic beads and anti-EIF4A3 (Proteintech, China) or anti-IgG (ABclonal, China). The enriched RNAs were analyzed by quantitative real-time PCR.
Wang X., Chen M, & Fang L. (2021). hsa_circ_0068631 promotes breast cancer progression through c-Myc by binding to EIF4A3. Molecular Therapy. Nucleic Acids, 26, 122-134.
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6

RNA Immunoprecipitation of circRPPH1

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RNA-Binding Protein Immunoprecipitation Kit (BersinBio, China) was used for conducting RNA immunoprecipitation (RIP) assay in MDA-MB-231 cells overexpressing circRPPH1 according to the manufacturer’s instructions. Anti-Ago2 (Abclonal, China) and Anti-IgG (Abclonal, China) were utilized for immunoprecipitation. The obtained RNAs were further analyzed by RT-qPCR.
Huang Y., Zheng W., Ji C., Wang X., Yu Y., Deng X., Zhou X, & Fang L. (2021). Circular RNA circRPPH1 promotes breast cancer progression via circRPPH1-miR-512-5p-STAT1 axis. Cell Death Discovery, 7, 376.
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