For intracellular cytokine analysis, cells were stimulated with PMA (50 ng/ml) and ionomycin (500 ng/ml) for 4 h in the presence of GolgiPlug (BD Pharmingen) and subsequently incubated with Live/Dead stain. Samples were then blocked with anti-FcγRIII/II and stained with αCD4 and αTCR-β. Cells were permeabilized and fixed using a Foxp3/Transcription Factor Fix/Perm kit (eBioscience) and stained by using fluorochrome-conjugated Abs according to the manufacturer's protocol.
Foxp3 transcription factor fix perm kit
Manufactured by Thermo Fisher Scientific
The Foxp3/Transcription Factor Fix/Perm kit is a laboratory tool designed for the intracellular staining and detection of Foxp3, a transcription factor. The kit provides the necessary reagents and protocols for the fixation, permeabilization, and staining of cells to enable the analysis of Foxp3 expression using flow cytometry or other suitable techniques.
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Lab products found in correlation
2 protocols using foxp3 transcription factor fix perm kit
1
Intracellular Cytokine Analysis Protocol
2
Intracellular Cytokine Analysis Protocol
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