Phycoerythrin conjugated pe mouse anti human cd29

To analyze the cell surface marker expression in RFP- and Oct4/Sox2-ATMSCs, flow cytometry was performed. By passage 5, a homogenous population of rapidly dividing cells with fibroblastoid morphology was obtained. ATMSCs were fixed with 70% ethanol at 4 °C and stained for 30 min on ice with primary antibodies that recognize various surface molecules. Phycoerythrin-conjugated (PE) mouse anti-human CD29 (BD Biosciences, San Jose, CA, USA), FITC (fluorescein isothiocyanate-conjugated) mouse anti-human CD31 (BD Biosciences), PE mouse anti-human CD34 (BD Biosciences), FITC mouse anti-human CD44 (BD Biosciences), FITC mouse anti-human CD45 (BD Biosciences), PE mouse anti-human CD73 (BD Biosciences Pharmingen, San Diego, CA, USA), PE mouse anti-human CD90 (BD Biosciences), and PE anti-human CD105/endoglin (R&D system, Minneapolis, MN, USA) were used for cell surface antigen detection. The immunophenotype of MSCs was analyzed using a FACS Calibur flow cytometer (BD Biosciences, Bedford, MA, USA) with CELLQuest software (BD Biosciences).