Hrp conjugated anti rabbit na934v and anti mouse nxa931 antibodies

Manufactured by GE Healthcare

The HRP-conjugated anti-rabbit (NA934V) and anti-mouse (NXA931) antibodies are secondary antibodies used in various immunoassay techniques. The anti-rabbit antibody is conjugated with the enzyme horseradish peroxidase (HRP), while the anti-mouse antibody is also HRP-conjugated. These antibodies are designed to bind to primary antibodies raised in rabbit or mouse, respectively, and can be utilized for the detection and visualization of target proteins or antigens in samples.

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Lab products found in correlation

Ecl system rpn2106, by GE Healthcare (2 mentions) Sodium bicarbonate 25080, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 without nahco3 51800, by Thermo Fisher Scientific (2 mentions) Dry milk 170 6404, by Bio-Rad (2 mentions) Pvdf membranes rpn303f, by GE Healthcare (2 mentions) Verteporfin, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) β actin actb, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

Close spelling variants of this product

Anti-rabbit-HRP (NA934V Anti-rabbit NA934V Anti-mouse NXA931 HRP-conjugated antibodies NXA931 NA934V HRP anti-mouse

2 protocols using hrp conjugated anti rabbit na934v and anti mouse nxa931 antibodies

Protein Extraction and Western Blotting

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RPMI-1640 (SH30255.01), trypsin (SH40003.12), phosphate-buffered saline (PBS, SH40003.12) and Fetal Bovine Serum (FBS) (SV30160.03) were purchased from HyClone. Sodium Bicarbonate (25080) and RPMI-1640 without NaHCO₃ (51800) were purchased from Gibco. Bafilomycin A1 (BafA1, B1793), protease cocktail tablets EDTA-free, phosphatase inhibitors (P5726, P0044) and Verteporfin were purchased from Sigma. Protein assay dye reagent concentrate (500-0006), protein assay standard (5000-0007) and dry milk (170-6404) were purchased from Bio-Rad. The following antibodies were used: LC3B (Cell Signaling Technology, 2775), SQTM1/p62 (BD Biosciences, 610833) and β-actin (Sigma, A5441). HRP-conjugated anti-rabbit (NA934V) and anti-mouse (NXA931) antibodies, ECL system (RPN2106) and PVDF membranes (RPN303F) were purchased from GE Healthcare. The fluorogenic substrate Suc-LLVY-AMC (S-280) was purchased from Boston Biochem (USA).

Pellegrini P., Serviss J.T., Lundbäck T., Bancaro N., Mazurkiewicz M., Kolosenko I., Yu D., Haraldsson M., D’Arcy P., Linder S, & De Milito A. (2018). A drug screening assay on cancer cells chronically adapted to acidosis. Cancer Cell International, 18, 147.

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Autophagy Regulation in Cell Lines

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RPMI-1640 (SH30255.01), trypsin (SH40003.12), and phosphate-buffered saline (PBS, SH40003.12) were from HyClone. RPMI-1640 without NaHCO₃ (51800) and Sodium Bicarbonate (25080) were from Gibco. BafA1 (B1793), CQ diphosphate salt (C6628), HCQ (H0915), protease cocktail tablets EDTA-free, phosphatase inhibitors (P5726, P0044) and bovine serum albumin (A7906) were from Sigma. The Lys-01 was synthesized by OncoTargeting AB (Sweden) and dissolved in DMSO. Protein assay standard I (5000-0007) and dry milk (170-6404) were from Bio-Rad. The following antibodies were used: LC3B (Cell Signaling Technology, 2775), cathepsin B (CTSB) (Calbiochem, PC41) and β-actin (ACTB) (Sigma, A5441). HRP-conjugated anti-rabbit (NA934V) and anti-mouse (NXA931) antibodies, ECL system (RPN2106) and PVDF membranes (RPN303F) were from GE Healthcare.

Pellegrini P., Strambi A., Zipoli C., Hägg-Olofsson M., Buoncervello M., Linder S, & De Milito A. (2014). Acidic extracellular pH neutralizes the autophagy-inhibiting activity of chloroquine: Implications for cancer therapies. Autophagy, 10(4), 562-571.

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