Tc2n rabbit polyclonal antibody

Manufactured by Abcam

Sourced in United States

The TC2N rabbit polyclonal antibody is a research-use-only product designed to detect the TC2N protein. It is a purified antibody raised in rabbits against a synthetic peptide corresponding to a region within the human TC2N protein sequence.

Automatically generated - may contain errors

Lab products found in correlation

Actin monoclonal antibody, by Merck Group (2 mentions) Cleaved caspase 3 rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions) Akt mouse monoclonal antibody, by Santa Cruz Biotechnology (1 mentions) P21 mouse monoclonal antibody, by Santa Cruz Biotechnology (1 mentions) P53 mouse monoclonal antibody, by Santa Cruz Biotechnology (1 mentions) Bcl 2 mouse monoclonal antibody, by Santa Cruz Biotechnology (1 mentions) Bax mouse monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)

2 protocols using tc2n rabbit polyclonal antibody

Immunoblotting of Signaling Proteins

Cited 1 time

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WB was performed as previously described^{27 (link)}. The following primary antibodies were used: TC2N rabbit polyclonal antibody (1:500; Abcam), p85 mouse monoclonal antibody (1:1000; Santa Cruz), p-p85 Tyr508 goat polyclonal antibody (1:1000; Santa Cruz), p-p55γ Tyr199 rabbit polyclonal antibody (1:1000; Bioss), p55γ mouse monoclonal antibody (1:1000; Santa Cruz), AKT mouse monoclonal antibody (1:1000; Santa Cruz), p-AKT Ser473 mouse monoclonal antibody (1:1000; Santa Cruz) GSK3B mouse monoclonal antibody (1:1000; Santa Cruz), p-GSK3B mouse monoclonal antibody (1:1000; Santa Cruz), p-MYC mouse monoclonal antibody (1:1000; Santa Cruz), p-BAD mouse monoclonal antibody (1:1000; Santa Cruz), Caspase-3 mouse monoclonal antibody (1:1000; Santa Cruz), cleaved-Caspase-3 rabbit monoclonal antibody (1:1000; Cell Signaling Technology), ALK mouse monoclonal antibody (1:1000; Santa Cruz), EBP1 mouse monoclonal antibody (1:1000; Santa Cruz) and ACTIN monoclonal antibody (1:2000; Sigma). ACTIN served as a loading control.

Hao X.L., Gao L.Y., Deng X.J., Han F., Chen H.Q., Jiang X., Liu W.B., Wang D.D., Chen J.P., Cui Z.H., Ao L., Cao J, & Liu J.Y. (2019). Identification of TC2N as a novel promising suppressor of PI3K-AKT signaling in breast cancer. Cell Death & Disease, 10(6), 424.

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Analysis of p53 Signaling Proteins

Cited 1 time

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WB was performed as previously described [50 (link)]. ACTIN was used as a loading control. The following primary antibodies were used: TC2N rabbit polyclonal antibody (1:500; Abcam), p53 mouse monoclonal antibody (1:1000; Santa Cruz Biotechnology), p21 mouse monoclonal antibody (1:1000; Santa Cruz Biotechnology), BAX mouse monoclonal antibody (1:1000; Santa Cruz Biotechnology), Bcl-2 mouse monoclonal antibody (1:1000; Santa Cruz Biotechnology), Cdk5 mouse monoclonal antibody (1:1000; Santa Cruz Biotechnology), phosphor-p53 (Ser-15) rabbit polyclonal antibody (1:500; Bioss), phosphor-p53 (Ser-33) rabbit polyclonal antibody (1:500; Bioss), phosphor-p53 (Ser-46) rabbit polyclonal antibody (1:500; Bioss), ubiquitin rabbit polyclonal antibody (1:500; Abcam) and ACTIN monoclonal antibody (1:2000; Sigma).
For co-immunoprecipitation, cell lysates were prepared and immunoprecipitated with indicated antibody or mouse IgG using the Co-IP Kit (Pierce, Rockford, IL, USA) according to the manufacturer’s instructions. The precipitated protein was assessed by WB using indicated antibody.

Hao X.L., Han F., Zhang N., Chen H.Q., Jiang X., Yin L., Liu W.B., Wang D.D., Chen J.P., Cui Z.H., Ao L., Cao J, & Liu J.Y. (2018). TC2N, a novel oncogene, accelerates tumor progression by suppressing p53 signaling pathway in lung cancer. Cell Death and Differentiation, 26(7), 1235-1250.

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