Apc anti human cd20

The stored Human peripheral blood mononuclear cells (PBMCs) were first treated with following steps. The PBMCs were quickly thawed in a 37 °C water bath. The suspensions were centrifuged at 500 × g for 5 min to remove the supernatant. The PBMCs were resuspended with 2% FACS buffer (phosphate buffered saline containing 2% fetal bovine serum). The PBMCs washed with 2% FACS buffer were filtered by Corning (Falcon) 100 μm cell strainers. After the pretreatment, the PBMCs were incubated with human Fc block (BioLegend) in the dark at 4 °C for 15 min. The PBMCs were then incubated with APC-cy7 anti-human CD3 (BioLegend), APC anti-human CD20 (BioLegend), FITC anti-human CD19 (BioLegend) and spike (S) protein-biotin in the dark at 4 °C for 30 min. The cells were then incubated with PerCP/Cyanine5.5 Streptavidin (BioLegend) and PE Streptavidin (BioLegend) in the dark at 4 °C for 30 min.