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6 protocols using 2 methylimidazole

1

Electrochemical Sensing of Biomolecules

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Phosphate buffer saline (PBS, 0.1 M, pH 7.0) was purchased from Source Leaf Biotechnology (Shanghai, China). Potassium ferricyanide (K3Fe(CN)6, ≥99.5%), isopropyl alcohol (≥99.7%) and potassium chloride (KCl, 99.5%) were provided by Aladdin (Shanghai, China). Dopamine hydrochloride (DA) and uric acid (UA, ≥99%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). l-Ascorbic acid (AA, >99%) citric acid (CA, 99.5%), l-cysteine (l-Cys, 99%), sodium carbonate (Na2CO3, 99.5%), calcium chloride (CaCl2, 99.99%), and magnesium sulfate (MgSO4, 99.5%) were bought from Macklin (Shanghai, China). Ethanol, zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99%), cobalt nitrate hydrate (Co(NO3)2·6H2O, 99%), dibasic sodium phosphate (Na2HPO4) and 2-methylimidazole (C4H6N2, 98%) were obtained from Adamas (Shanghai, China). All of the chemicals were used without further purification. The water used in the experiments was obtained from a Milli-Q system (18.2 MΩ cm−1).
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2

Synthesis and Characterization of Fluorescent Nanoparticles

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The following chemicals were obtained from Chron Chemicals: NaCl (AR, 99.5%), citric acid (AR), D‐glucose monohydrate (AR), zinc acetate dihydrate (AR), sodium hydroxide (AR, 98%). Tartrazine (85%), tetraethyl orthosilicate (98%), and ammonia solution (28% in water) were purchased from Aladdin Industrial Corporation. Indigo Carmine (≥70%) and 2‐methylimidazole (98%) were obtained from Adamas beta. Trichloro (1H,1H,2H,2H‐Perfluorooctyl) Silane (97%) was purchased from Sigma Aldrich. Hemoglobin was obtained from Bioss. Deionized water with a resistivity of 18.5 MΩ cm was used for the measurement.
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3

Synthesis of Metal-Organic Frameworks

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Iron(III) chloride hexahydrate (FeCl3·6H2O, 99.0%), methanol (CH3OH, AR), and cetyltrimethylammonium bromide (C19H42BrN, 99%) were purchased from Sigma-Aldrich Chemical Reagent Co., Ltd. Zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99.0%), 2-methylimidazole (C4H6N2, 98.0%), and 2-aminobenzene-1,4-dicarboxylic acid (NH2-BDC, 98.0%) were obtained from Adamas Reagent Co. Ltd. Nafion D-520 dispersion (5 wt.%) was purchased from Dupont China holding. Commercial 20 wt.% and 40 wt.% Pt/C was purchased from Shanghai Hesen Electric Co., LTD. All chemicals were used without further purification.
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4

Synthesis of Metal-Organic Frameworks

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Iron(III) chloride hexahydrate (FeCl3·6H2O, 99.0%), methanol (CH3OH, AR), and cetyltrimethylammonium bromide (C19H42BrN, 99%) were purchased from Sigma-Aldrich Chemical Reagent Co., Ltd. Zinc nitrate hexahydrate (Zn(NO3)2·6H2O, 99.0%), 2-methylimidazole (C4H6N2, 98.0%), and 2-aminobenzene-1,4-dicarboxylic acid (NH2-BDC, 98.0%) were obtained from Adamas Reagent Co. Ltd. Nafion D-520 dispersion (5 wt.%) was purchased from Dupont China holding. Commercial 20 wt.% and 40 wt.% Pt/C was purchased from Shanghai Hesen Electric Co., LTD. All chemicals were used without further purification.
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5

Synthesis of Cobalt-Silica Nanocomposites

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All chemicals and solvents were used directly without further purification. Ammonium hydroxide (NH4OH, AR, 25–28 wt%), ethanol (AR), sodium hydroxide (NaOH, AR) were acquired from Sinopharm Chemical Reagent Co. Ltd (China). Tetraethoxysilane (TEOS, 99%), 3-hydroxytyramine hydrochloride (dopamine, 99%), cobalt nitrate hexahydrate (Co(NO3)2·6H2O, 99%), 2-methylimidazole (2-MI, 98%) were purchased from Adamas Reagent, Ltd.
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6

Synthesis of Microporous Metal-Organic Frameworks

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Materials.
Hexadecyltrimethylammonium bromide (CTAB) and Hexadecyltrimethylammonium chloride (CTAC) were purchased from Aladdin; HCl were purchased from Adamas; 2-methylimidazole (HMIM), Zinc nitrate hexahydrate (Zn(NO 3 ) 2 •6H 2 O), Gold(III) chloride hydrate, Tris(hydroxymethyl)aminomethane (TRIS), silver nitrate and NaCl were purchased from Sigma-Aldrich.
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