19091s 433e hp5ms

The TE flower extract was analyzed using gas chromatography coupled to mass (GS-MS) using an Agilent Technologies 5975C VL MSD, Santa Clara, CA, USA. The silica column used was Agilent 19091s-433E HP5MS, Santa Clara, CA, USA (30 m × 0.25 mm, 0.25 µm film thickness). The gas used was Helium, with a constant flow of 2.0 mL/min. Manual injection volume was 0.1 µL (Separation ratio: 33.4:1). The injector and ion source temperatures were set at 260 °C. The mass spectrum was taken at intervals of 2.5 min; the mass range was from 30 to 500 Da. Fragmentation was compared to the computer library (Adams, IL, USA, 2005).

GC-MS analysis was carried out using an Agilent GC-MS 7890A/5975C series instrument with a 30 m×0.32 mm×0.25 µm capillary column (19 091S-433E HP-5MS; Agilent Technologies). Helium was the carrier gas with a flow velocity of 1 ml min⁻¹ and 1 µl of sample was injected into the column in a splitless mode. The analytical conditions were an initial temperature of 50 °C, with isothermal operation for 1 min followed by heating to 120 °C at a constant rate of 20 °C min⁻¹ and a final heating to 310 °C at a constant rate of 4 °C min⁻¹ [49 (link)] with a 5 min isothermal operation. The column was directly connected to an electron ionisation mass spectrometer with an electron energy of 70 eV, producing ions that are characterised according to mass-to-charge ratio and relative abundance.