Dsc 204 f1 phoenix differential scanning calorimeter

All chemical spectral analyses were conducted at Kuwait University Research Center. Thin layer chromatography (TLC) was performed using Polygram SIL G/UV 254 TLC plates, and the results were visualized under ultraviolet light at 254 and 350 nm, with hexane/ethyl acetate as a solvent. Column chromatography was performed using silica gel 60A with a mesh size of 40–60 µm. ¹H and ¹³C nuclear magnetic resonance (NMR) spectra were obtained using a Bruker DPX 600 NMR spectrophotometer at 600 MHz and 150 MHz in DMSO and CDCL3, respectively. Mass spectra were detected on a GC-MS DFS–Thermo spectrometer. An IR spectrum was obtained with a Jasco 6300 FTIR spectrometer. Melting point was determined using a Netzsch DSC 204 F1 Phoenix differential scanning calorimeter.
SKOV3 human ovarian cancer cells were obtained from American Type Culture Collection. A cholesterol-conjugated HSP27 inhibitor was synthesized in our laboratory. Cell culture media and other supplements, such as 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), were obtained from Sigma-Aldrich (Milwaukee, WI). Chemicals and reagents are commercially available and ready for direct use, requiring no preparation. Biological parameters were examined at the Biology Department, Kuwait University.

The physicochemical property of NOR-PC was characterized by differential scanning calorimetry (DSC) and Fourier transform infrared spectrophotometry (FT-IR). DSC analyses of NOR, phospholipid, NOR-PC, and physical mixture of NOR and phospholipid were recorded using a Netzsch DSC 204 F1 phoenix differential scanning calorimeter (Netzsch, Selb, Germany), respectively. The samples were heated from 40°C to 300°C at a heating rate of 10°C/min. The FT-IR spectra of the examined samples were conducted in the range of 4000–400 cm⁻¹ with a resolution of 4 cm⁻¹ using a Bruker tensor 27 FT-IR spectrometer (Bruker Corporation, Billerica, MA, USA). The examined samples were obtained employing compressed disks of appropriate amount of NOR, phospholipid, NOR-PC, and physical mixture of NOR and phospholipid separately mixed with KBr in the dry environment.