Easysep cd11b positive selection kit

Manufactured by STEMCELL

The EasySep CD11b Positive Selection Kit is a laboratory tool used for the isolation and enrichment of CD11b-positive cells from various sample types. It utilizes magnetic particles and a specialized buffer system to selectively target and separate CD11b-expressing cells from the rest of the cell population. The isolated cells can then be used for further analysis or downstream applications.

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Lab products found in correlation

L glutamine solution, by Wisent (1 mentions) Mem amino acid solution, by Wisent (1 mentions) Penicillin streptomycin solution, by Wisent (1 mentions) Amphotericin b solution, by Wisent (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dmem f12, by Wisent (1 mentions) 100 μm cell strainer, by BD (1 mentions) Caco 2, by American Type Culture Collection (1 mentions) Poly d lysine coated, by BD (1 mentions) Thp 1 tib 202 cells, by American Type Culture Collection (1 mentions) F4 80 pe cy5 bm8, by Thermo Fisher Scientific (1 mentions) Cyan adp 9, by Beckman Coulter (1 mentions) Gr 1 fitc rb6 8c5, by BD (1 mentions) Kaluza, by Beckman Coulter (1 mentions) Moflo astrio, by Beckman Coulter (1 mentions) Cytofix cytoperm kit, by BD (1 mentions)

Close spelling variants of this product

EasySepTM Human CD11b positive selection Kit EasySep™ Mouse CD11b Positive Selection Kit EasySep Mouse CD11b Positive Selection Kit II EasySep kits

3 protocols using easysep cd11b positive selection kit

Isolation of Primary Microglia from Neonatal Rat Cortices

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Cortices from 1-day-old pups were extracted and placed onto a 100-mm petri dish using aseptic techniques. Cortices were sliced with a commercial razor blade, further broken up with a rigorous up-and-down motion in 10 mL of medium, and filtered with a 70-μm filter. The cells were then plated onto a 100-mm petri dish and put in an incubator of 37 °C with 5 % CO₂. Cell culture medium DMEM/F12 (Wisent, St. Bruno, QC) was supplemented with 10 % FBS (Invitrogen, Burlington, ON), 1 % penicillin-streptomycin solution (Wisent, St-Bruno, QC), 1 % L-glutamine solution (Wisent, St. Bruno, QC), 0.9 % sodium pyruvate solution (Wisent, St. Bruno, QC), 0.9 % MEM amino acid solution (Wisent, St. Bruno, QC), and 0.9 % amphotericin B solution (Wisent, St. Bruno, QC). The medium of the mixed glial culture was changed every 2 to 3 days. After 3 weeks, primary microglia cells were separated from astrocytes using EasySep CD11b positive selection kit following the manufacturer’s instructions (Stem cell, Vancouver, BC).

Gharagozloo M., Mahvelati T.M., Imbeault E., Gris P., Zerif E., Bobbala D., Ilangumaran S., Amrani A, & Gris D. (2015). The nod-like receptor, Nlrp12, plays an anti-inflammatory role in experimental autoimmune encephalomyelitis. Journal of Neuroinflammation, 12, 198.

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Murine Myeloid and Human Monocytic Cells

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Primary murine myeloid cells flushed from femurs were isolated from female mice aged ~5 weeks, passed through a 100 μm-cell strainer (BD Falcon) and purified using the EasySep CD11b Positive Selection Kit (Stemcell). Murine bone-marrow derived CD11b⁺-myeloid cells were cultured on Poly-D-Lysine-coated (BD BioCoat) tissue culture plastic in Leibovitz’s L-15 medium (Life) supplemented with 10% v/v FBS (Thermo or PAA) and penicillin/streptomycin (Life) in an air incubator at 37°C. Human CD11b⁺-myeloid (34 (link)) monocytic-like THP-1 cells (TIB-202; passages #13-#19) were obtained from the American Type Culture Collection (ATCC) and maintained in RPMI1640 medium (PAA or Life) supplemented with 10% v/v FBS, 2 mM L-glutamine (Life) and penicillin/streptomycin in a humidified incubator at 37°C with 5% CO₂. The intestinal epithelial cell line Caco-2 (HTB-37; #11-#15) was purchased from ATCC and maintained as described previously (35 (link)).

Frank M., Hennenberg E.M., Eyking A., Rünzi M., Gerken G., Scott P., Parkhill J., Walker A.W, & Cario E. (2015). TLR signaling modulates side effects of anticancer therapy in the small intestine. Journal of immunology (Baltimore, Md. : 1950), 194(4), 1983-1995.

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VSV-Mediated Cytokine Induction in EMT6 Cells

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EMT6 cells were cotreated with 0.1 MOI of VSVΔ51-GFP and 5 μM LCL161 for 20 hr. Cells were trypsinized, permeabilized with the CytoFix/CytoPerm kit (BD Biosciences) and stained with APC-TNFα (MP6-XT22, BD Biosciences). Cells were analyzed on a Cyan ADP 9 flow cytometer (Beckman Coulter) and data was analyzed with FlowJo (Tree Star).
Splenocytes were enriched for CD11b using the EasySep CD11b positive selection kit (StemCell Technologies). CD49⁺ cells were enriched using the EasySep CD49b positive selection kit (StemCell Technologies) from the CD11b⁻ fraction. CD11b⁺ cells were stained with F4/80-PE-Cy5 (BM8, eBioscience) and Gr1-FITC (RB6-8C5, BD Biosciences) and further sorted with MoFlo Astrios (Beckman Coulter). Flow cytometry data was analyzed using Kaluza (Beckman Coulter). Isolated cells were infected with VSVΔ51 for 24 hr and clarified cell culture supernatants was applied to EMT6 cells for 24 hr in the presence of 5 μM LCL161. An n = 3 of biological replicates was used to determine statistical measures (mean, standard deviation).

Beug S.T., Tang V.A., LaCasse E.C., Cheung H.H., Beauregard C.E., Brun J., Nuyens J.P., Earl N., St-Jean M., Holbrook J., Dastidar H., Mahoney D.J., Ilkow C., Le Boeuf F., Bell J.C, & Korneluk R.G. (2014). Smac mimetics and innate immune stimuli synergize to promote tumor death. Nature biotechnology, 32(2), 182-190.

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