Total RNA was extracted from snap-frozen pellets of CBO at day 25, 50, 100, 150, 200, fetal cortical progenitors and fetal brain tissues using the Rneasy Mini Kit (Qiagen, 74104). Purified RNA was quantified using a NanoDrop spectrophotometer and RNA quality was checked with an Agilent 2100 Bioanalyzer using the RNA nano kit (Agilent, 5067-1512). Library preparation for RNA sequencing was performed according to TruSeq Total RNA sample preparation protocol (Illumina, RS-122-2202), starting from 250 ng to 1 μg of total RNA. cDNA library quality was assessed with an Agilent 2100 Bioanalyzer, using the high-sensitivity DNA kit (Agilent 5067-4626). Libraries were sequenced with the Illumina Novaseq machine at a read length of 50 bp paired-end and a coverage of 35 million of reads per sample.
Truseq total rna sample preparation protocol
Manufactured by Illumina
The TruSeq Total RNA sample preparation protocol is a laboratory equipment designed to enable efficient and reliable preparation of total RNA samples for sequencing. This protocol provides a standardized workflow for isolating and purifying total RNA from a variety of sample types, including cells, tissues, and other biological sources. The core function of this product is to facilitate the extraction and processing of total RNA samples in a consistent and reproducible manner, enabling downstream genetic analysis and applications.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Rna nano kit, by Agilent Technologies (1 mentions)
High sensitivity dna kit, by Agilent Technologies (1 mentions)
Nanodrop spectrophotometer, by Agilent Technologies (1 mentions)
Novaseq machine, by Illumina (1 mentions)
Hiseq machine, by Illumina (1 mentions)
2 protocols using truseq total rna sample preparation protocol
1
Transcriptome Analysis of Cortical Development
2
RNA-seq Library Preparation and Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Library preparation for RNA-seq was performed according to the TruSeq Total RNA sample preparation protocol (Illumina), starting from 250 ng to 1 μg of total RNA. cDNA library quality was assessed in an Agilent 2100 Bioanalyzer using the High Sensitivity DNA Kit. Libraries were sequenced with the Illumina HiSeq machine at a read length of 50–base pair (bp) paired end and a coverage of 35 million of reads per sample.
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