Blood samples were collected from WT and Osx-Nf1 KO mice at sacrifice. Vitamin
D, phosphate and calcium concentration in mouse serum was determined using a
25OH-Vitamin-D ELISA Assay kit (Eagle Biosciences, cat# VID31-K01), a Phosphate
Assay kit (BioVision, cat # k410–500) and a
Calcium Assay kit (BioVision,
cat# k380–250), respectively, according to the manufacturer’s
instructions.
de la Croix Ndong J., Makowski A.J., Uppuganti S., Vignaux G., Ono K., Perrien D.S., Joubert S., Baglio S.R., Granchi D., Stevenson D.A., Rios J.J., Nyman J.S, & Elefteriou F. (2014). Asfotase-α improves bone growth, mineralization and strength in mouse models of neurofibromatosis type-1. Nature medicine, 20(8), 904-910.