Oligotex mrna spin column kit

Manufactured by Qiagen

Sourced in United States

The Oligotex mRNA Spin-Column Kit is a laboratory tool designed for the isolation of messenger RNA (mRNA) from various sample types, including cells and tissues. The kit utilizes oligo(dT) coated magnetic beads to selectively capture and purify mRNA molecules from total RNA extracts.

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Lab products found in correlation

Smart cdna library construction kit, by Takara Bio (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Biophotometer, by Eppendorf (1 mentions) Pcr selecttm cdna subtraction kit, by Takara Bio (1 mentions)

2 protocols using oligotex mrna spin column kit

cDNA Library Construction from Jellyfish Tentacle

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2.1. cDNA Library Construction
Total RNA was extracted from the tentacle of C. capillata with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), and then, the mRNA was isolated using the Oligotex mRNA Spin-Column Kit (Qiagen, Valencia, CA, USA). The concentration of purified mRNA was determined at 260 nm using a BioPhotometer (Eppendorf, Hamburg, Germany). The cDNA library was constructed using the SMART cDNA Library Construction Kit (Clontech, Mountain View, CA, USA), according to the manufacturer’s instructions.

Ruan Z., Liu G., Wang B., Zhou Y., Lu J., Wang Q., Zhao J, & Zhang L. (2014). First Report of a Peroxiredoxin Homologue in Jellyfish: Molecular Cloning, Expression and Functional Characterization of CcPrx4 from Cyanea capillata. Marine Drugs, 12(1), 214-231.

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RNA Extraction and cDNA Synthesis

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Total RNA from time 0 h samples and time 24 h-shreds was isolated following the hot borate method29 (link). Isolation of poly A⁺ mRNA from total RNA was performed using the Oligotex mRNA Spin-Column kit (Qiagen). Purified poly A⁺ mRNA from time 0 h samples and time 24 h-shreds were used for cDNA synthesis by the PCR-Select^TM cDNA subtraction kit (Clontech).

Jacobo-Velázquez D.A., González-Agüero M, & Cisneros-Zevallos L. (2015). Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response. Scientific Reports, 5, 8608.

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