Maldi calibrants

Manufactured by Merck Group

Sourced in United States

MALDI calibrants are a series of standard reference materials used for calibrating and optimizing matrix-assisted laser desorption/ionization (MALDI) mass spectrometers. They provide accurate mass references to ensure precise mass measurements during MALDI-MS analysis.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Nutrient broth, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Thermo Fisher Scientific (1 mentions) Sinapinic acid, by Merck Group (1 mentions) Serratia marcescens, by Carolina Biological Supply (1 mentions) Absolute ethanol, by Thermo Fisher Scientific (1 mentions) Trifluoroacetic acid, by Thermo Fisher Scientific (1 mentions) α cyano 4 hydroxycinnamic acid, by Thermo Fisher Scientific (1 mentions) Formic acid, by Merck Group (1 mentions)

2 protocols using maldi calibrants

Bacterial Strain Cultivation and Characterization

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Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 132), and Serratia marcescens (ATCC 13880) were purchased from Carolina Biological Supply Company (Burlington, NC, USA) and stored as freezer stock cultures (nutrient broth:glycerol, 87.5∶12.5) at −80 °C.
Sinapinic acid was purchased from Sigma-Aldrich (St. Louis, MO, USA). Trifluoroacetic acid (TFA) and acetonitrile were purchased from ACROS (Fair Lawn, NJ, USA). MALDI calibrants were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nutrient agar and nutrient broth were purchased from Carolina Biological Supply Company (Burlington, NC, USA).

Zhang L., Borror C.M, & Sandrin T.R. (2014). A Designed Experiments Approach to Optimization of Automated Data Acquisition during Characterization of Bacteria with MALDI-TOF Mass Spectrometry. PLoS ONE, 9(3), e92720.

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Mass Spectrometry Sample Preparation

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Acetonitrile (≥99.7%) was purchased from Alfa Aesar (Ward Hill, MA, USA). Trifluoroacetic acid (≥99.5%) and α-cyano-4-hydroxycinnamic acid (CHCA) (≥97%) were purchased from ACROS (Fair Lawn, NJ, USA). Formic acid (≥88.0%) and MALDI calibrants were purchased from Sigma-Aldrich (St. Louis, MO, USA). Absolute ethanol was purchased from Thermo Fisher Scientific (Waltham, MS, USA). R2A agar was purchased from Carolina Biological Supply Company (Burlington, NC, USA).

Zhang L., Smart S, & Sandrin T.R. (2015). Biomarker- and similarity coefficient-based approaches to bacterial mixture characterization using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Scientific Reports, 5, 15834.

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