Mastic gum

Mastic gum, carrageenan, xanthan gum, guar gum, gum tragacanth, locust bean gum, and β-glucan were purchased from Sigma Aldrich (Saint Louis, MO, USA). Extracts from these items were prepared according to the procedures described by Vojdani [27 ]. Ten grams of each gum was extracted in 500 mL of buffer pH 4.6 by mixing them for 8 hours at 25°C on a magnetic stirrer. The solution was centrifuged at 20,000g, and supernatant was removed and concentrated by a factor of 10 using an Amicon filter. The protein concentration was measured using a kit provided by Bio-Rad (Hercules, CA, USA). All extracts were aliquoted and stored frozen at −20°C until used. Different gum extracts were dissolved in 0.1 M PBS. These antigens were diluted 1 : 50 in 0.1 M carbonate buffer pH 9.2 and 100 μL of each gum antigen.

Mastic gum, carrageenan, xanthan gum, guar gum, gum tragacanth, locust bean gum, and β-glucan were purchased from Sigma Aldrich (Saint Louis, MO, USA). Ten grams of each gum were extracted in 500 mL of buffer pH 4.6 by mixing them for 8 hours at 25°C on a magnetic stirrer. The solution was centrifuged at 20,000g, and supernatant was removed and concentrated by a factor of 10 using an Amicon filter. The protein concentration was measured using a kit provided by Bio-Rad (Hercules, CA, USA). All extracts were aliquoted and stored frozen at −20°C until used.