Securin

Manufactured by GeneTex

Sourced in United States

SECURIN is a laboratory equipment designed to secure and protect sensitive samples. It provides a secure environment for storing and transporting delicate materials, ensuring their integrity and safety.

Automatically generated - may contain errors

Lab products found in correlation

α tubulin, by Bio-Rad (2 mentions) Aurkb, by BD (2 mentions) Cyclin a and b, by Santa Cruz Biotechnology (2 mentions) Phosphohistone h 3 ph 3, by Merck Group (1 mentions)

2 protocols using securin

Mitotic Arrest Dynamics Evaluation

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Example 10

Phospho-histone-H3-488 (Ser10) (p-H3-488, Cell Signaling), α-tubulin (Serotec), AurKB (BD Transduction), Anti-Centromere-Antibodies (ACA, Cortex Biochem), cyclin A and B (Santa Cruz Biotechnology), and SECURIN (Gene Tex). BubR1 and Bub1 were from Hongtao Yu. FITC-, Cy3- and Cy5-conjugated secondary antibodies were from Jackson Immuno Research.

Cells were synchronized in G1/S, released into the cell cycle in the presence of DMSO or MI-181, and cell extracts were prepared at several time points post-release. Immoblot analysis of these extracts revealed that MI-181 treated cells arrested in mitosis (p-H3 positive), activated the SAC (BubR1 remained phosphorylated) and stabilized cyclin B while degrading cyclin A. (FIG. 8). Additionally, the MI-182-induced mitotic arrest was reversible, as cells exited mitosis within 2 hours of drug washout (FIG. 9).

US10913750B2. Tubulin-binding compounds, compositions and uses related thereto (2021-02-09). The Regents of the University of California [US]. Inventors: Jorge Torres [US], Robert Damoiseaux [US], Todd O. Yeates [US], Silvia Senese [US], Dan E. McNamara [US], Yu-Chen Lo [US].

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Mitotic Checkpoint Protein Visualization

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Phospho-histone-H3-488 (Cell Signaling), Phospho-histone-H3 (p-H3, Millipore, Billerica, MA, USA) α-tubulin (Serotec, Raleigh, NC, USA), AurKB (BD Transduction, San Jose, CA, USA), Anti-Centromere-Antibodies (ACA, Cortex Biochem, Madison, WI, USA), cyclin A and B (Santa Cruz Biotechnology, Dallas, TX, USA), and SECURIN (Gene Tex, Irvine, CA, USA). BubR1 and Bub1 were from Hongtao Yu. FITC-, Cy3-, and Cy5-conjugated secondary antibodies were from Jackson Immuno Research.

Senese S., Lo Y.C., Huang D., Zangle T.A., Gholkar A.A., Robert L., Homet B., Ribas A., Summers M.K., Teitell M.A., Damoiseaux R, & Torres J.Z. (2014). Chemical dissection of the cell cycle: probes for cell biology and anti-cancer drug development. Cell Death & Disease, 5(10), e1462-.

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