Anti vcam 1 fitc

Manufactured by BD

Anti-VCAM-1 FITC is a fluorescently labeled antibody that binds to the cell surface protein VCAM-1 (Vascular Cell Adhesion Molecule-1). VCAM-1 is involved in cell-cell adhesion and is expressed on the surface of endothelial cells.

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Lab products found in correlation

Polyethylenimine (pei), by Merck Group (2 mentions) Anti cd8 pacific blue, by BD (2 mentions) Anti cxcr6 fitc, by R&D Systems (2 mentions) Anti cx3cl1 fitc, by R&D Systems (2 mentions) Anti e selectin pe, by BD (2 mentions) Cyan adp flow cytometer, by Beckman Coulter (2 mentions) Anti cd19 pe cy7, by BD (1 mentions) Anti cd14 allophycocyanin, by BD (1 mentions) Anti cd14 apc, by BD (1 mentions) Anti icam 1 apc, by BD (1 mentions)

2 protocols using anti vcam 1 fitc

Multicolor Flow Cytometry Panel

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PBLs and monocytes were stained with anti-CD3 peridinin-chlorophyll proteins–Cy5.5, anti-CD4 allophycocyanin-Cy7, anti-CD8 Pacific Blue, anti-CD14 allophycocyanin, and anti-CD19 PE-Cy7 (BD Biosciences), and anti-CX3CR1 FITC and anti-CXCR6 FITC (R&D Systems). HUVECs were stained with anti-CX3CL1 FITC and anti-CXCL16 allophycocyanin (R&D Systems). As a positive control for these transmembrane chemokines, HEK-293T cells were transfected with expression constructs for human CX3CL1 (8 (link)) or CXCL16 (7 (link)), using polyethylenimine (Sigma) as described (27 ). HUVECs were also stained with anti-E-selectin PE, anti-ICAM-1 allophycocyanin and anti-VCAM-1 FITC (BD Biosciences). Cells were analyzed using a Cyan ADP Flow Cytometer (Beckman Coulter) and FlowJo software (Ashland). Live cells were gated based on forward and side scatter parameters.

Reyat J.S., Chimen M., Noy P.J., Szyroka J., Rainger G.E, & Tomlinson M.G. (2017). ADAM10-Interacting Tetraspanins Tspan5 and Tspan17 Regulate VE-Cadherin Expression and Promote T Lymphocyte Transmigration. The Journal of Immunology Author Choice, 199(2), 666-676.

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Characterization of Immune Cell Phenotypes

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PBLs and monocytes were stained with anti-CD3 peridinin-chlorophyll proteins (PerCP)-Cy5.5, anti-CD4 allophycocyanin (APC)-Cy7, anti-CD8 Pacific Blue, anti-CD14 APC and anti-CD19 phycoerythrin (PE)-Cy7 (BD Biosciences), and anti-CX3CR1 fluorescein isothiocyanate (FITC) and anti-CXCR6 FITC (R&D Systems). HUVECs were stained with anti-CX3CL1 FITC and anti-CXCL16 APC (R&D Systems). As a positive control for these transmembrane chemokines, HEK-293T cells were transfected with expression constructs for human CX3CL1 (8 (link)) or CXCL16 (7 (link)), using polyethylenimine (Sigma) as described (27 ). HUVECs were also stained with anti-E-selectin PE, anti-ICAM-1 APC and anti-VCAM-1 FITC (BD Biosciences). Cells were analysed using a Cyan ADP Flow Cytometer (Beckman Coulter) and FlowJo software (Ashland). Live cells were gated based on forward and side scatter parameters.

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