Tartrate-resistant acid phosphatase (TRAP) staining was performed on paraffin sections of knees from P0, P8, P14 and 2-month-old mice. The sections were incubated in Basic Stock Incubation Solution (BSIS, Sodium acetate, Sodium tartrate and Acetic acid, pH 4.7–5.0) with Napthol AS-BI phosphate dissolved in 2-Ethoxyethanol at 37 °C degree for 1 h. The samples were then rinsed in BSIS with Sodium Nitrite and Pararosaniline dye and developed at 37 °C until the desired intensity of the red staining in osteoclasts was achieved. The samples were counterstained with 0.02% Fast Green for 2.5 min. TRAP+ cells at the COJ were counted using a Nikon Labophot microscope (Tokyo, Japan) at 200× magnification on at least 2 sections per animal.
Labophot microscope
Manufactured by Nikon
Sourced in Japan
The Labophot microscope is a laboratory instrument designed for high-quality optical microscopy. It features a sturdy, ergonomic construction and provides clear, detailed images for various scientific and research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using labophot microscope
1
Quantifying Osteoclasts at Cartilage-Bone Junction
2
Visualizing Chromocenters in Artemia Nuclei
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The interphase nuclei were obtained from nauplii by the squash method following the Colihueque and Gajardo (1996) (link) protocol. Larvae were collected either from newly hatched cysts incubated in artificial seawater or from offspring of natural crosses of adults reared in the laboratory under standardised conditions of salinity (35%), temperature (~22 ºC) and light (~1000 lux). The chromocenters of the nuclei were stained using a fluorescent dye (Hoechst 33258) which displays a high affinity for interphase heterochromatic regions (Latt and Wohlled, 1975) (link). The nuclei were photographed at 1000x using a 7 mpx digital camera mounted on an epifluorescence Nikon Labophot microscope. Before taking the photographs, excitation of the fluorochrome was undertaken with UV light through an appropriate filter (UV-2A, 330-380 nm). Five nuclei of each nauplii were photographed at random, totaling from 20 to 58 nuclei per population.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!