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Ab202894

Manufactured by Proteintech
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Ab202894 is a laboratory antibody product provided by Proteintech. It is designed for use in various research applications. The core function of this product is to serve as a tool for specific protein detection and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Facsaria 3 cell sorter, by BD (2 mentions) Pcmvr8, by Addgene (2 mentions) Pcmv vsv g, by Addgene (2 mentions) Lenti x cells, by Takara Bio (2 mentions) A8592, by Merck Group (2 mentions) Lentiviral vectorbackbone, by Addgene (2 mentions)

2 protocols using ab202894

1

Generation of ALYREF Overexpressing Cell Lines

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The production of the cell line was previously described in ref
28 (link). Generation of
cell lines overexpressing FLAG-ALYREF. Wildtype ALYREF or mutant
ALYREFM-c+ΔdcDNA and a N-terminal 3xFlag tag were cloned into a lentiviral vector
backbone (Addgene plasmid #31485), yielding a plasmid containing pRRL-SFFV
3x-Flag-Aly/REF-p2a-mCherry. For an overexpression of ALYREF variants in
K562 THOC1-GFP overexpressing cells, lentiviral particles carrying the
3x-Flag-ALYREF constructs were generated using Lenti-X cells (Takara) by
polyethylenimine transfection (Polysciences) of the viral plasmid and helper
plasmids pCMVR8.74 (Addgene plasmid #22036) and pCMV-VSV-G (Addgene plasmid
#8454), according to standard procedures. THOC1-GFP overexpressing K562
(DSMZ) cells were infected at limiting dilutions and GFP and mCherry double
positive cells were isolated using a BD FACSAria III cell sorter (BD
Biosciences). Viral integration was confirmed by immunoblotting for ALYREF
(antibodies: Ancam ab202894 and Proteintech 16690-1-AP) and FLAG (A8592;
Sigma-Aldrich).
Pacheco-Fiallos B., Vorländer M.K., Riabov-Bassat D., Fin L., O’Reilly F.J., Ayala F.I., Schellhaas U., Rappsilber J, & Plaschka C. (2023). mRNA recognition and packaging by the human transcription-export complex. Nature, 616(7958), 828-835.
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2

Generation of ALYREF Overexpressing Cell Lines

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The production of the cell line was previously described in ref
28 (link). Generation of
cell lines overexpressing FLAG-ALYREF. Wildtype ALYREF or mutant
ALYREFM-c+ΔdcDNA and a N-terminal 3xFlag tag were cloned into a lentiviral vector
backbone (Addgene plasmid #31485), yielding a plasmid containing pRRL-SFFV
3x-Flag-Aly/REF-p2a-mCherry. For an overexpression of ALYREF variants in
K562 THOC1-GFP overexpressing cells, lentiviral particles carrying the
3x-Flag-ALYREF constructs were generated using Lenti-X cells (Takara) by
polyethylenimine transfection (Polysciences) of the viral plasmid and helper
plasmids pCMVR8.74 (Addgene plasmid #22036) and pCMV-VSV-G (Addgene plasmid
#8454), according to standard procedures. THOC1-GFP overexpressing K562
(DSMZ) cells were infected at limiting dilutions and GFP and mCherry double
positive cells were isolated using a BD FACSAria III cell sorter (BD
Biosciences). Viral integration was confirmed by immunoblotting for ALYREF
(antibodies: Ancam ab202894 and Proteintech 16690-1-AP) and FLAG (A8592;
Sigma-Aldrich).
Pacheco-Fiallos B., Vorländer M.K., Riabov-Bassat D., Fin L., O’Reilly F.J., Ayala F.I., Schellhaas U., Rappsilber J, & Plaschka C. (2023). mRNA recognition and packaging by the human transcription-export complex. Nature, 616(7958), 828-835.
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