Cellstar 96 well culture microtiter plates

Cellstar 96-well culture microtiter plates, 10 and 50 mL tubes were from Greiner (Alphen a/d Rijn, the Netherlands). Sheath fluid (FM3D) and drive fluid (MAGPIX) were both purchased from Luminex (Austin, USA). The following mycotoxins and metabolites were purchased from Biopure (Tulln, Austria): FB₁, FB₂, FB₃, OTA, OTB, AFB₁, aflatoxin B₂ (AFB₂), aflatoxin G₁ (AFG₁), aflatoxin G₂ (AFG₂), aflatoxin M1 (AFM₁), T-2, HT-2, DON, D3G, 3-acetyl-DON (3ADON), 15-acetyl-DON (15ADON), NIV, ZEN, α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL). Zearalenone-14-β-D-glucopyranoside (Z14G), α-zearalenol-14-β-D-glucopyranoside (α-ZELG), β-zearalenol-14-β-D-glucopyranoside (β-ZELG) and zearalenone 14-sulfate (Z14S) were produced according to [52 ] or isolated from Fusarium inoculated rice. Syringeless filter devices (Mini-UniPrep, PTFE) for sample clean-up were purchased from GE Healthcare (Rotterdam, the Netherlands). Acetonitrile (ACN) and methanol (MeOH) were purchased from Biosolve (Valkenswaard, the Netherlands), formic acid (FA) from Merck (Amsterdam, the Netherlands) and ammonium formate (AMF) from Fluka Analytical (Steinheim, Germany). All other chemicals were purchased from VWR International (Amsterdam, the Netherlands).

Drive fluid and the paramagnetic color-encoded microsphere sets 043 and 038 were obtained from Luminex. Cellstar 96-well culture microtiter plates were purchased from Greiner (Alphen a/d Rijn, the Netherlands). The βCA2 and βCA1 proteins and respective IgY antibodies were purchased from GeneTel Laboratories LLC (Madison, WI, USA). The secondary reporter antibody, Goat anti-chicken coupled to R-Phycoerythrin (GAC-RPE), was obtained from immuno Reagents Inc. (Raleigh, NC, USA). The 2-(N-morpholino)ethanesulfonicacid (MES), N-hydroxysulfosuccinimide (sulfo-NHS), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich (Zwijndrecht, the Netherlands). Phosphate buffered saline (PBS), and sodium phosphate monobasic (NaH₂PO₄∙H₂O) were obtained from Merck (Darmstadt, Germany).