Anti-NLRP3 (#ab91413), anti-caspase-1 (#ab1872), anti-IL-1β(#ab9722), anti-GluA1 (#ab31232), anti-GluA2 (#ab133477), anti-sodium potassium ATPase (#ab76020) and anti-VCP/p97 (#ab11433) antibodies were purchased from Abcam. Anti-β-actin antibody (#A5441) was obtained from Sigma-Aldrich. Anti-PSD-95 antibody (#MAB1598) was obtained from Millipore.
The Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (#SM-005) was purchased from Invent Biotechnologies, Inc. Roche Applied Science provided Complete Protease Inhibitor Cocktail Tablets (#04693116001). The Pierce™ BCA Protein Assay Kit (#23225) was purchased from Thermo Scientific. Protein A/G Magnetic Beads (#B23202) was purchased from Bimake. AC-YVAD-CMK peptide (#SML0429) was purchased from Sigma-Aldrich. For the in vitro experiments, cultured primary neurons were pretreated with AC-YVAD-CMK (5 µM) 1 h before oxygen-glucose deprivation (OGD). For the in vivo experiments, AC-YVAD-CMK (1 mg/kg, i.p.) was administered 1 h before HIBD surgery and then daily for 7 days.
The Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (#SM-005) was purchased from Invent Biotechnologies, Inc. Roche Applied Science provided Complete Protease Inhibitor Cocktail Tablets (#04693116001). The Pierce™ BCA Protein Assay Kit (#23225) was purchased from Thermo Scientific. Protein A/G Magnetic Beads (#B23202) was purchased from Bimake. AC-YVAD-CMK peptide (#SML0429) was purchased from Sigma-Aldrich. For the in vitro experiments, cultured primary neurons were pretreated with AC-YVAD-CMK (5 µM) 1 h before oxygen-glucose deprivation (OGD). For the in vivo experiments, AC-YVAD-CMK (1 mg/kg, i.p.) was administered 1 h before HIBD surgery and then daily for 7 days.