Api yeast identification system

Yeasts were grown aerobically at 37°C on Sabouraud agar with 0.4 g/l chloramphenicol and 0.04 g/l gentamycin (BD Diagnostics, Franklin Lakes, NJ, USA). All in vitro investigations were conducted on a third subculture of C. albicans ATCC 10231 (Oxoid; Thermo Fisher Scientific, Inc., Waltham, MA, USA) suspended in Sabouraud broth (cat. no. CM147; Oxoid™; Thermo Fisher Scientific, Inc.) or in distilled water. The suspension was adjusted to 1–20×106 blastoconidia per ml by dilution, following a blastoconidia count using a Thoma cell counting chamber (Marienfeld™, Lauda-Königshofen, Germany). Wild strains were isolated by swabbing from dentures and identified on the basis of their colony aspect on CHROMagar™ medium (BD Diagnostics), by chlamydoconidia formation on BT™ Rice Extract agar (BD Diagnostics) and by the API yeast identification system (bioMérieux, Marcy-l'Etoile, France).

Yeasts were aerobically grown at 37°C for 24 hours in Sabouraud broth (OXOID™ CM147; Basingstoke, UK) or on solid medium, such as Sabouraud agar with chloramphenicol and gentamicin or CHROMagar™ media (BD Diagnostics™, Erembodegem, Belgium). The investigations were carried out on C. albicans ATCC 10231 (Culti-Loops™, Thermo Scientific™, Lenexa, KS, USA), which is often chosen to test antifungals,15 (link) and on clinical strains sampled from dentures using sterile cotton swabs (EUROTUBO^®; Deltalab™, Barcelona, Spain). Clinical isolates were identified according to the appearance of their colonies on CHROMagar™ medium, by chlamydoconidia formation on Rice Extract agar with Polysorbate 80 (BD Diagnostics™) and using the API yeast identification system (bioMérieux, Marcy-l’Etoile, France). All in vitro investigations were performed in a third subculture on Sabouraud solid medium. Candida suspensions were adjusted in phosphate buffer (0.1 M pH 7.4) to 10⁶ (or 2×10⁷) blastoconidia per mL by dilution after counting in a Thoma cell chamber (Marienfeld™, Lauda-Königshofen, Germany).