Patu8988t

Human pancreatic cancer cell lines BxPC-3, PANC-1, Patu8988T and AsPC-1 were obtained from Shanghai Institute of Biosciences and Cell Resources Center (Chinese Academy of Sciences, Shanghai, China). AsPC-1 and BxPC-3 cells were grown in McCoy’s 5A medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco), PANC-1 and Patu8988T cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 media (Gibco) with 10% FBS. All the cells were cultured in a humidified cell incubator with an atmosphere of 5% CO₂ at 37 °C.

Human pancreatic cancer cell lines (PANC-1 and Patu8988T) were obtained from Shanghai Institute of Biosciences and Cell Resources Center (Chinese Academy of Sciences, Shanghai, China). PANC-1 and Patu8988T cells were grown in Roswell Park Memorial Institute (RPMI)-1640 media (Gibco) with 10% FBS, 100 units/mL penicillin, and 100 μg/ml streptomycin at 37 C in a humidified incubator with 5% CO₂.
Erlotinib and Alantolactone were obtained from Aladdin (Shanghai, China). PLGA (38,000 MW) was bought from Jinan Daigang Biological Engineering Co. Ltd (Jinan, China). Poly (vinylalcohol) (PVA; 20,000–30,000 MW) was purchased from Acros Organics (New Jersey, USA). DSPE-PEG2000 was bought from AVT (Shanghai) Pharmaceutical Co., Ltd. Methyl thiazolyl tetrazolium (MTT) was obtained from Aladdin (Shanghai) Biochemical and Technology Co., Ltd. The antibodies against p-STAT3, STAT3, β-Actin, Bax, Bcl-2 were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies of p-EGFR, EGFR was purchased from Santa Cruz Biotechnology Inc (Dallas, TX, USA). The horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and HRP-conjugated goat anti-rabbit IgG were obtained from Biosharp Biotechnology. Other chemicals or reagents were of analytical grade.