Optical rotations were recorded on a JASCO P-2000 polarimeter with a 1.0 mL cell. UV spectra were measured on an UV-Vis spectrophotometer (UV/EV300). IR spectra were recorded using an IR/Nicolet 6700 Fourier transform infrared spectrometer. The NMR data were collected by a Bruker Avance III 600 MHz spectrometer at 600 MHz for 1H nuclei and 150 MHz for 13C nuclei. Mass spectra were measured on a positive ion mode using LC/HRMS with a Waters ACQUITY UPLC system coupled with a Waters Micromass Q-TOF Premier Mass Spectrometer, which was equipped with an electrospray interface. Preparative medium-phased liquid chromatography (MPLC) was performed on a flash purification system (Bonna Agela Technologies Corporation, Tianjin, China). High Performance Liquid Chromatography (HPLC) was carried out on an Agilent 1200 liquid chromatography system equipped with a DAD detector. (R)-(−)/(S)-(+)-α-MPA, 4-dimethylaminopyridine (DMAP), and N,N′-dicyclohexylcarbodiimide (DCC) were purchased from J&K Scientific Corporation (Beijing, China).
N n dicyclohexylcarbodiimide dcc
Manufactured by J&K Scientific
Sourced in China
N,N′-dicyclohexylcarbodiimide (DCC) is a coupling agent commonly used in organic synthesis. It is a white crystalline solid that facilitates the formation of amide bonds between carboxylic acids and amines.
Automatically generated - may contain errors
Lab products found in correlation
P 2000 polarimeter, by Jasco (1 mentions)
4 dimethylaminopyridine dmap, by J&K Scientific (1 mentions)
Agilent 1200 liquid chromatography system, by Agilent Technologies (1 mentions)
Avance 3 600 mhz spectrometer, by Bruker (1 mentions)
Micromass q tof premier mass spectrometer, by Waters Corporation (1 mentions)
Acquity uplc system, by Waters Corporation (1 mentions)
Dna marker, by Tiangen Biotech (1 mentions)
Taq pcr mastermix, by Tiangen Biotech (1 mentions)
N fmoc amino acids, by GL Biochem (1 mentions)
3 full race core set ver 2.0 kit, by Takara Bio (1 mentions)
Methanol, by J&K Scientific (1 mentions)
3 protocols using n n dicyclohexylcarbodiimide dcc
1
Spectroscopic Characterization of Chiral Compounds
2
Peptide Synthesis and Animal Experiments
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The pGEM-T Easy Vector System, E. coli. Dh5α, 2×Taq PCR Master Mix, and DNA Marker were obtained from TIANGEN Biotech Co., Ltd. (Beijing, China). The 3′-Full RACE Core Set Ver.2.0 kit was obtained from TaKaRa (Dalian, China). The Nucleic Acid purification kit was purchased from Dongsheng Biotech (Guangzhou, China). N-Fmoc-amino acids and HOBt were sourced from GL Biochem Ltd. (Shanghai, China). Rink resin was purchased from Tianjin Nankai Hecheng S&T Co. (Tianjin, China). N,N′-Dicyclohexylcarbodiimide (DCC) and Methanol were purchased from J&K Chemical Ltd. (Shanghai, China) and Honeywell Burdick & Jackson (Muskegon, MI United States), respectively. The other reagents were of analytical grade.
Adult male Sprague-Dawley rats (SD, 250 g, Beijing Animal Center, China) were housed in groups of eight and maintained on a 12 h light-dark cycle (light cycle from 8 am to 8 pm) at a temperature of 23 ± 2°C and relative humidity of 50%. Food pellets and water were available ad libitum. All experiments were conducted in accordance with the guidelines of the Beijing Institutes for Biological Sciences Animal Research Advisory Committee and conformed to the European Community directives for the care and use of laboratory animals.
Adult male Sprague-Dawley rats (SD, 250 g, Beijing Animal Center, China) were housed in groups of eight and maintained on a 12 h light-dark cycle (light cycle from 8 am to 8 pm) at a temperature of 23 ± 2°C and relative humidity of 50%. Food pellets and water were available ad libitum. All experiments were conducted in accordance with the guidelines of the Beijing Institutes for Biological Sciences Animal Research Advisory Committee and conformed to the European Community directives for the care and use of laboratory animals.
3
Synthesis of Genistein-Loaded Hydrogel
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Methyl ether poly(ethylene glycol) (MPEG, Mn = 4000) and 3-amino-1-propanol (AP) were purchased from TCI (Tokyo, Japan). HA (Mn = 6800) obtained from Bloomage Freda Biopharm (Shandong, China). 1,6-hexanediol diacrylate (HDD), 4-dimethylaminopyridine (DMAP), and N,N′-dicyclohexylcarbodiimide (DCC) were acquired from J&K Scientific LTD (Beijing, China). Genistein was purchased from Selleck.cn (Shanghai, China).
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