Apc conjugated anti mouse cd80

Manufactured by Thermo Fisher Scientific

Sourced in China, United States

The APC-conjugated anti-mouse CD80 is a flow cytometry reagent that binds to the CD80 molecule expressed on the surface of mouse immune cells. It can be used to identify and analyze cells expressing CD80 in mouse samples.

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2 protocols using apc conjugated anti mouse cd80

Comprehensive Immune Cell Profiling

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Phycoerythrin (PE)/allophycocyanin (APC)–conjugated anti-mouse CD9, PE/APC-conjugated anti-human CD31, PE-conjugated anti-mouse CD62P, fluorescein isothiocyanate (FITC)–conjugated anti-mouse CD11c, APC/Cy7-conjugated anti-mouse CD3, BV510-conjugated anti-mouse CD3, eFluor 450/PE–conjugated anti-mouse CD8a, APC-conjugated anti-mouse CD80, APC/Cy7-conjugated anti-mouse CD86, and peridinin chlorophyll protein (PerCP) Cy5.5–conjugated anti-mouse CD62L antibodies were purchased from eBioscience (Beijing, China). Advanced RPMI 1640 medium, Dulbecco’s modified Eagle’s medium (DMEM), and fetal bovine serum (FBS) were obtained from Gibco (Beijing, China). Adjuvant R837 hydrochloride, HAuCl₄, AgNO₃, NaBH₄, resveratrol, and cetyl trimethylammonium bromide (CTAB) were purchased from Sigma-Aldrich (Shanghai, China). PBS, Hepes solution, and enzyme-linked immunosorbent assay (ELISA) kit were purchased from Solarbio (Beijing, China). Other chemicals were purchased from J&K (Beijing, China) and used without purification.

Lv Y., Li F., Wang S., Lu G., Bao W., Wang Y., Tian Z., Wei W, & Ma G. (2021). Near-infrared light–triggered platelet arsenal for combined photothermal-immunotherapy against cancer. Science Advances, 7(13), eabd7614.

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Multiparametric Flow Cytometry Analysis

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For cell surface and intracellular marker analysis, 10⁶ cells were incubated with fluorescent-conjugated antibodies in labeling solution (eBioscience, USA). The fluorescent-conjugated antibodies used in this study included PE-conjugated anti-mouse CD11c, FITC-conjugated anti-mouse CD11c, FITC-conjugated anti-mouse CD86, FITC-conjugated anti-mouse MHC-II, APC-conjugated anti-mouse CD80, PE-CY5-conjugated anti-mouse CD40, APC-conjugated anti-mouse CD4, FITC-conjugated anti-mouse IL-17A, FITC-conjugated anti-mouse Foxp3, PE-conjugated anti-mouse GATA-3, PE-conjugated anti-mouse T-bet antibodies, and FITC-conjugated anti-mouse CD103 (integrin alpha E) (all purchased from eBioscience, USA). Fluorescent-conjugated, isotype-matched, irrelevant antibodies were used to establish background fluorescein levels. Flow cytometry analysis was conducted on a FACSCalibur (BD Biosciences, USA), and FACSCalibur software (BD Biosciences) was used to analyze the flow data. DCs were gated for PE-CD11c, and then FITC-MHC-II expression and endocytic FITC-OVA levels were analyzed. MLR-lymphocytes were gated for APC-CD4, and then FITC-Foxp3 was analyzed.

Tang H.C., Lai Y.Y., Zheng J., Jiang H.Y, & Xu G. (2020). miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob. Journal of Immunology Research, 2020, 1379458.

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