ChIP-grade antibodies were αH3.3K27me3 (custom antibody made by Proteintech), αH3.3 (Invitrogen RM190), αH3K27me3 (Active Motif 39155), αH3 (Abcam 1791), rabbit IgG (Abcam 171870) (Fig. S5 ). Additional antibodies used for western blotting (WB) included αH3K27me3 (Abcam 6002), αREST (EMD Millipore 07–579), αH3K4me3 (Abcam 8580), αH3K27ac (Active Motif 39133), and αH3K9me3 (Abcam 8898).
αh3k27me3
Manufactured by Active Motif
The αH3K27me3 is an antibody that specifically recognizes the trimethylated lysine 27 on histone H3 (H3K27me3). This histone modification is associated with transcriptional repression and plays a role in the regulation of gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 700 confocal microscope, by Zeiss (2 mentions)
αh3k27ac, by Active Motif (1 mentions)
α h3k9me3, by Abcam (1 mentions)
αh3k4me3, by Abcam (1 mentions)
α h3k27me3, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Cell Signaling Technology (1 mentions)
α pmad, by Abcam (1 mentions)
α h3k9me3, by Active Motif (1 mentions)
α h3k4me1, by Active Motif (1 mentions)
α h3k4me3, by Active Motif (1 mentions)
2 protocols using αh3k27me3
1
Chromatin Immunoprecipitation Antibodies
2
Immunostaining of Drosophila Imaginal Discs
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Imaginal discs were dissected and fixed in 4% paraformaldehyde for 20 min, washed and permeabilized in phosphate-buffered saline with 0.1% Triton X-100, and blocked in 10% normal goat serum. Primary antibodies used were α-Ci [1:25; Developmental Studies Hybridoma Bank (DSHB)], α-Ubx (1:10; DSHB), α-En (1:25; DSHB), α-β-galactosidase (1:500; Promega), α-V5 (1:500; Sigma-Aldrich), α-Wg (1:100; DSHB), α-Cut (1:100), α-Ptc (1:50; DSHB), α-pMAD (1:500; Abcam), α-Smo (1:10; DSHB), α-MMP1 (1:100; a combination of 14A3D2, 3A6B4, and 5H7B11; DSHB), α-Tara (1:700; K. Koh), α-H3K27me3 (1:500; Active Motif), α-H3K9me3 (1:500; Active Motif), α-H3K4me1 (1:500; Active Motif), α-H3K4me3 (1:500; Active Motif), α-H4ac (1:500; Active Motif), and α-Sd (1:500; K. Guss). Secondary antibodies were from Cell Signaling Technology and used at 1:400. Nuclei were stained with 4′,6-diamidino-2-phenylindole (1:1000; Cell Signaling Technology). Samples were imaged on a Zeiss LSM 700 confocal microscope.
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