Protein extracts were transferred to polyvinylidene difluoride membranes as previously described (3 (link)). Membranes were blocked with Odyssey Blocking Buffer in tris-buffered saline (LI-COR) and incubated overnight at 4°C with the following primary antibodies: Hspa5 (1:2000; Proteintech, 11587-1-AP), Pdia6 (1:2000; Abcam, ab11432), Biotin Ligase Epitope Tag (for Tavi-tag detection, 1:1000; Abcam, ab106159), PSD95 (1:5000; Thermo Fisher Scientific, 6G6-1C9), hemagglutinin (HA) (1:2000; Millipore Sigma), phospho-tau AT8 (1:5000; BioLegend, 806503), actin (1:10,000; Thermo Fisher Scientific), ATF4 (1:500; Thermo Fisher Scientific, PA5-27576), ATF6 (1:500; Thermo Fisher Scientific, PA5-114886), and phospho-IRE1α (1:500; Thermo Fisher Scientific, PA5-85738). Membranes were washed and incubated with appropriate IRDye immunoglobulin G (IgG) secondary antibodies, including anti-rabbit IRDye 800LT (1:5000; LI-COR) and anti-mouse IRDye 680CW (LI-COR). Images were acquired using the Odyssey Infrared Imaging System (LI-COR). Quantification of Western blot bands was performed using Image Studio Lite v.5.2 (LI-COR).
Anti rabbit irdye 800lt
Manufactured by LI COR
The Anti-rabbit IRDye 800LT is a near-infrared fluorescent dye conjugated to an anti-rabbit antibody. It is designed for use in Western blotting and other immunoassay applications that require the detection of rabbit-derived proteins.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse irdye 680cw, by LI COR (2 mentions)
Odyssey infrared imaging system, by LI COR (2 mentions)
Phospho ire1α, by Thermo Fisher Scientific (1 mentions)
Hspa5, by Proteintech (1 mentions)
Odyssey blocking buffer, by LI COR (1 mentions)
Image studio lite v5, by LI COR (1 mentions)
Psd 95, by Thermo Fisher Scientific (1 mentions)
Pdia6, by Abcam (1 mentions)
Odyssey blocking buffer in tbs, by LI COR (1 mentions)
Image studio lite ver 5, by LI COR (1 mentions)
2 protocols using anti rabbit irdye 800lt
1
Western Blot Analysis of Protein Expression
2
Quantification of Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whole-cell lysates were run on a 4–20% Tris-HCl Protein Gel (BIO-RAD, # 3450033) and transferred to methanol-activated polyvinylidene difluoride membranes. Membranes were blocked with Odyssey® Blocking Buffer in TBS (LI-COR) and incubated overnight at 4 °C with the following primary antibodies: pan-HA (1:1000, Cell signaling), YFP (1:1000, Abcam), and Actin (1:10,000, ThermoFisher Scientific). Membranes were washed and incubated with anti-rabbit IRDye 800LT (1:5000, LI-COR, # 926-32211) and anti-mouse IRDye 680CW (1:5000, LI-COR, #926-68022). Images were acquired using the Odyssey Infrared Imaging System (LI-COR). Quantification of western blot bands was performed using Image Studio Lite ver5.2 (LI-COR).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!